دورية أكاديمية
Osmoprotection of Salmonella enterica serovar Typhimurium by Ngamma-acetyldiaminobutyrate, the precursor of the compatible solute ectoine.
| العنوان: | Osmoprotection of Salmonella enterica serovar Typhimurium by Ngamma-acetyldiaminobutyrate, the precursor of the compatible solute ectoine. |
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| المؤلفون: | García-Estepa R; Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain., Cánovas D, Iglesias-Guerra F, Ventosa A, Csonka LN, Nieto JJ, Vargas C |
| المصدر: | Systematic and applied microbiology [Syst Appl Microbiol] 2006 Dec; Vol. 29 (8), pp. 626-33. Date of Electronic Publication: 2006 Feb 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier GmbH Country of Publication: Germany NLM ID: 8306133 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0723-2020 (Print) Linking ISSN: 07232020 NLM ISO Abbreviation: Syst Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [Munich, Germany] : Elsevier GmbH Original Publication: Stuttgart ; New York : G. Fischer Verlag, c1983- |
| مواضيع طبية MeSH: | Water-Electrolyte Balance*, Amino Acids, Diamino/*metabolism , Aminobutyrates/*pharmacology , Salmonella typhimurium/*drug effects , Salmonella typhimurium/*physiology, Amino Acids, Diamino/pharmacology ; Aminobutyrates/isolation & purification ; Aminobutyrates/metabolism ; Chromatography, High Pressure Liquid ; Halomonadaceae/genetics ; Halomonadaceae/metabolism ; Heat-Shock Response ; Hydro-Lyases/genetics ; Magnetic Resonance Spectroscopy ; Mutation ; Osmolar Concentration ; Salmonella typhimurium/growth & development ; Salmonella typhimurium/metabolism ; Sodium Chloride ; Symporters |
| مستخلص: | N(gamma)-acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile Chromohalobacter salexigens DSM 3043(T) could alleviate the inhibitory effects of high salinity in S. enterica, which lacks the ectoine biosynthetic pathway. NADA, purified from extracts of the mutant, protected S. enterica against salinity stress. This osmoprotective effect was slightly lower than that of ectoine, but more potent than that of hydroxyectoine. Accumulation of purified NADA by S. enterica was demonstrated by (13)C-NMR spectroscopy and HPLC analysis. In addition, it was shown that NADA was taken up by S. enterica via the ProP and ProU transport systems, which are known to transport glycine betaine and proline. This finding provides evidence that these permeases can recognize a diaminoacid that carries an unsubstituted alpha-amino group. This is the first time that NADA has been connected with osmoprotective functions in non-halophilic bacteria. |
| المشرفين على المادة: | 0 (Amino Acids, Diamino) 0 (Aminobutyrates) 0 (N(gamma)-acetyl-2,4-diaminobutyric acid) 0 (Symporters) 0 (hydroxyectoine) 451W47IQ8X (Sodium Chloride) 7GXZ3858RY (ectoine) EC 4.2.1.- (Hydro-Lyases) EC 4.2.1.- (ectoine synthase) |
| تواريخ الأحداث: | Date Created: 20060214 Date Completed: 20070118 Latest Revision: 20151119 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.syapm.2006.01.008 |
| PMID: | 16469465 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0723-2020 |
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| DOI: | 10.1016/j.syapm.2006.01.008 |