التفاصيل البيبلوغرافية
| العنوان: |
DNA methylation profiles of donor nuclei cells and tissues of cloned bovine fetuses. |
| المؤلفون: |
Kremenskoy M; Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan., Kremenska Y, Suzuki M, Imai K, Takahashi S, Hashizume K, Yagi S, Shiota K |
| المصدر: |
The Journal of reproduction and development [J Reprod Dev] 2006 Apr; Vol. 52 (2), pp. 259-66. Date of Electronic Publication: 2006 Feb 10. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Japanese Society of Animal Reproduction Country of Publication: Japan NLM ID: 9438792 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0916-8818 (Print) Linking ISSN: 09168818 NLM ISO Abbreviation: J Reprod Dev Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Tokyo : Japanese Society of Animal Reproduction, |
| مواضيع طبية MeSH: |
DNA Methylation*, Cell Nucleus/*metabolism , Cloning, Organism/*methods, Animals ; Brain/metabolism ; Cattle ; Cloning, Molecular ; CpG Islands ; Epigenesis, Genetic ; Female ; Genetic Techniques ; Genome ; Mice ; Ovary/metabolism ; Placenta/metabolism |
| مستخلص: |
Methylation of DNA in CpG islands plays an important role during fetal development and differentiation because CpG islands are preferentially located in upstream regions of mammalian genomic DNA, including the transcription start site of housekeeping genes and are also associated with tissue-specific genes. Somatic nuclear transfer (NT) technology has been used to generate live clones in numerous mammalian species, but only a low percentage of nuclear transferred animals develop to term. Abnormal epigenetic changes in the CpG islands of donor nuclei after nuclear transfer could contribute to a high rate of abortion during early gestation and increase perinatal death. These changes have yet to be explored. Thus, we investigated the genome-wide DNA methylation profiles of CpG islands in nuclei donor cells and NT animals. Using Restriction Landmark Genomic Scanning (RLGS), we showed, for the first time, the epigenetic profile formation of tissues from NT bovine fetuses produced from cumulus cells. From approximately 2600 unmethylated NotI sites visualized on the RLGS profile, at least 35 NotI sites showed different methylation statuses. Moreover, we proved that fetal and placental tissues from artificially inseminated and cloned cattle have tissue-specific differences in the genome-wide methylation profiles of the CpG islands. We also found that possible abnormalities occurred in the fetal brain and placental tissues of cloned animals. |
| تواريخ الأحداث: |
Date Created: 20060214 Date Completed: 20060612 Latest Revision: 20190608 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1262/jrd.17098 |
| PMID: |
16474212 |
| قاعدة البيانات: |
MEDLINE |
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