دورية أكاديمية
A global assembly of cotton ESTs.
| العنوان: | A global assembly of cotton ESTs. |
|---|---|
| المؤلفون: | Udall JA; Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011, USA., Swanson JM, Haller K, Rapp RA, Sparks ME, Hatfield J, Yu Y, Wu Y, Dowd C, Arpat AB, Sickler BA, Wilkins TA, Guo JY, Chen XY, Scheffler J, Taliercio E, Turley R, McFadden H, Payton P, Klueva N, Allen R, Zhang D, Haigler C, Wilkerson C, Suo J, Schulze SR, Pierce ML, Essenberg M, Kim H, Llewellyn DJ, Dennis ES, Kudrna D, Wing R, Paterson AH, Soderlund C, Wendel JF |
| المصدر: | Genome research [Genome Res] 2006 Mar; Vol. 16 (3), pp. 441-50. Date of Electronic Publication: 2006 Feb 14. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1088-9051 (Print) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1995- |
| مواضيع طبية MeSH: | Expressed Sequence Tags*, Gossypium/*genetics, DNA, Complementary/genetics ; Diploidy ; Gene Expression Profiling/methods ; Genome, Plant ; Molecular Sequence Data ; Polyploidy ; Sequence Analysis, DNA |
| مستخلص: | Approximately 185,000 Gossypium EST sequences comprising >94,800,000 nucleotides were amassed from 30 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including drought stress and pathogen challenges. These libraries were derived from allopolyploid cotton (Gossypium hirsutum; A(T) and D(T) genomes) as well as its two diploid progenitors, Gossypium arboreum (A genome) and Gossypium raimondii (D genome). ESTs were assembled using the Program for Assembling and Viewing ESTs (PAVE), resulting in 22,030 contigs and 29,077 singletons (51,107 unigenes). Further comparisons among the singletons and contigs led to recognition of 33,665 exemplar sequences that represent a nonredundant set of putative Gossypium genes containing partial or full-length coding regions and usually one or two UTRs. The assembly, along with their UniProt BLASTX hits, GO annotation, and Pfam analysis results, are freely accessible as a public resource for cotton genomics. Because ESTs from diploid and allotetraploid Gossypium were combined in a single assembly, we were in many cases able to bioinformatically distinguish duplicated genes in allotetraploid cotton and assign them to either the A or D genome. The assembly and associated information provide a framework for future investigation of cotton functional and evolutionary genomics. |
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| المشرفين على المادة: | 0 (DNA, Complementary) |
| تواريخ الأحداث: | Date Created: 20060216 Date Completed: 20060918 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC1415220 |
| DOI: | 10.1101/gr.4602906 |
| PMID: | 16478941 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1088-9051 |
|---|---|
| DOI: | 10.1101/gr.4602906 |