دورية أكاديمية
Transfer of vancomycin resistance transposon Tn1549 from Clostridium symbiosum to Enterococcus spp. in the gut of gnotobiotic mice.
| العنوان: | Transfer of vancomycin resistance transposon Tn1549 from Clostridium symbiosum to Enterococcus spp. in the gut of gnotobiotic mice. |
|---|---|
| المؤلفون: | Launay A; Centre d'Etudes Pharmaceutiques, Châtenay-Malabry, France., Ballard SA, Johnson PD, Grayson ML, Lambert T |
| المصدر: | Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2006 Mar; Vol. 50 (3), pp. 1054-62. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 0315061 Publication Model: Print Cited Medium: Print ISSN: 0066-4804 (Print) Linking ISSN: 00664804 NLM ISO Abbreviation: Antimicrob Agents Chemother Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology |
| مواضيع طبية MeSH: | DNA Transposable Elements* , Germ-Free Life*, Clostridium symbiosum/*genetics , Digestive System/*microbiology , Enterococcus/*genetics , Vancomycin Resistance/*genetics, Animals ; Anti-Bacterial Agents/pharmacology ; Base Sequence ; Clostridium symbiosum/drug effects ; Clostridium symbiosum/growth & development ; Colony Count, Microbial ; Conjugation, Genetic ; DNA, Bacterial/genetics ; DNA, Circular/genetics ; Drug Resistance, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Enterococcus/drug effects ; Enterococcus/growth & development ; Enterococcus faecalis/drug effects ; Enterococcus faecalis/genetics ; Enterococcus faecalis/growth & development ; Enterococcus faecium/drug effects ; Enterococcus faecium/genetics ; Enterococcus faecium/growth & development ; Feces/microbiology ; Genes, Bacterial ; Mice ; Molecular Sequence Data ; Vancomycin/pharmacology |
| مستخلص: | The vancomycin resistance vanB2 gene cluster is disseminated worldwide and has been found in phylogenetically remote bacterial genera. The vanB2 operon is part of conjugative transposons Tn1549/Tn5382, but conjugative transposition of these elements has not been demonstrated. We have obtained transfer of a Tn1549-like element (referred to herein as "Tn1549-like") from Clostridium symbiosum MLG101 to Enterococcus faecium 64/3 and Enterococcus faecalis JH2-2 in the digestive tract of gnotobiotic mice and to E. faecium 64/3 in vitro. Retransfer of Tn1549-like from an E. faecium transconjugant also containing Tn916 to E. faecium BM77 was obtained in vitro, albeit at a very low frequency. Transfer efficiency was found to be both donor and recipient dependent. Pulsed-field gel electrophoresis analysis of total SmaI-digested DNA of 48 transconjugants indicated in 27 instances the acquisition of ca. 34 kb of DNA. Two transconjugants harbored two copies of the transposon. Sequencing of the flanking regions of Tn1549-like in 48 transconjugants revealed 29 integration events in 26 loci in the E. faecium genome, and two hot spots for insertion were identified. Integration of the transposon was associated with the acquisition of 5 (n = 18) or 6 (n = 7) bp of donor DNA or with 5-bp duplications of target DNA in the remaining transconjugants. These data demonstrate functionality of the Tn1549-like element and attest that the transfer of the vanB operon between enterococci and human commensal anaerobes occurs in the intestinal environment. |
| References: | Lancet. 2001 Mar 17;357(9259):855-6. (PMID: 11265957) Antimicrob Agents Chemother. 2005 May;49(5):1688-94. (PMID: 15855482) Antimicrob Agents Chemother. 2003 Feb;47(2):786-9. (PMID: 12543693) Science. 2003 Mar 28;299(5615):2071-4. (PMID: 12663927) J Clin Microbiol. 2003 Apr;41(4):1499-506. (PMID: 12682136) Antimicrob Agents Chemother. 2003 Aug;47(8):2579-83. (PMID: 12878522) Antimicrob Agents Chemother. 2003 Sep;47(9):2993-6. (PMID: 12937011) Appl Environ Microbiol. 2004 Feb;70(2):758-64. (PMID: 14766552) J Bacteriol. 1974 Feb;117(2):360-72. (PMID: 4204433) J Bacteriol. 1985 Jun;162(3):1212-20. (PMID: 2987186) N Engl J Med. 1988 Jul 21;319(3):157-61. (PMID: 2968517) Antimicrob Agents Chemother. 1990 Aug;34(8):1565-9. (PMID: 2171424) Antimicrob Agents Chemother. 1991 Jan;35(1):185-7. (PMID: 1849709) J Bacteriol. 1991 Nov;173(22):7136-41. (PMID: 1657880) J Bacteriol. 1992 Apr;174(8):2582-91. (PMID: 1556077) J Bacteriol. 1993 Jan;175(1):117-27. (PMID: 8380148) Mol Microbiol. 1993 Apr;8(1):179-85. (PMID: 8388531) Mol Microbiol. 1994 Mar;11(6):1099-108. (PMID: 8022279) FEMS Microbiol Lett. 1994 Jun 15;119(3):359-63. (PMID: 8050717) Genomics. 1995 Feb 10;25(3):674-81. (PMID: 7759102) J Antimicrob Chemother. 1995 Oct;36(4):595-606. (PMID: 8591934) Antimicrob Agents Chemother. 2000 Mar;44(3):705-9. (PMID: 10681342) Microbiology. 2000 Jun;146 ( Pt 6):1481-9. (PMID: 10846226) Clin Microbiol Rev. 2000 Oct;13(4):686-707. (PMID: 11023964) Trends Microbiol. 1996 Oct;4(10):401-7. (PMID: 8899966) Antimicrob Agents Chemother. 1997 Jan;41(1):24-9. (PMID: 8980749) J Bacteriol. 1997 Jan;179(1):97-106. (PMID: 8981985) Antimicrob Agents Chemother. 1997 Apr;41(4):872-4. (PMID: 9087511) Antimicrob Agents Chemother. 1998 Jan;42(1):202-5. (PMID: 9449290) Antimicrob Agents Chemother. 1998 Apr;42(4):963-4. (PMID: 9559822) Mol Microbiol. 1998 Apr;28(1):103-17. (PMID: 9593300) Antimicrob Agents Chemother. 1998 Aug;42(8):1871-7. (PMID: 9687377) Antimicrob Agents Chemother. 1998 Aug;42(8):2055-9. (PMID: 9687406) J Bacteriol. 1998 Sep;180(17):4426-34. (PMID: 9721279) J Antimicrob Chemother. 1998 Aug;42(2):275-6. (PMID: 9738855) Antimicrob Agents Chemother. 1998 Sep;42(9):2215-20. (PMID: 9736537) Antimicrob Agents Chemother. 1999 May;43(5):1105-10. (PMID: 10223921) J Bacteriol. 1999 Sep;181(17):5414-8. (PMID: 10464215) Antimicrob Agents Chemother. 2004 Dec;48(12):4915-8. (PMID: 15561881) Antimicrob Agents Chemother. 2005 Jan;49(1):77-81. (PMID: 15616278) Antimicrob Agents Chemother. 2005 Jan;49(1):470-2. (PMID: 15616340) J Antimicrob Chemother. 2005 Apr;55(4):466-74. (PMID: 15731199) Plasmid. 2002 Sep;48(2):77-97. (PMID: 12383726) |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (DNA Transposable Elements) 0 (DNA, Bacterial) 0 (DNA, Circular) 6Q205EH1VU (Vancomycin) |
| تواريخ الأحداث: | Date Created: 20060224 Date Completed: 20060427 Latest Revision: 20181113 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC1426432 |
| DOI: | 10.1128/AAC.50.3.1054-1062.2006 |
| PMID: | 16495268 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0066-4804 |
|---|---|
| DOI: | 10.1128/AAC.50.3.1054-1062.2006 |