دورية أكاديمية
Dexamethasone can stimulate G1-S phase transition in human airway fibroblasts in asthma.
| العنوان: | Dexamethasone can stimulate G1-S phase transition in human airway fibroblasts in asthma. |
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| المؤلفون: | Fouty B; Center for Lung Biology, MSB 3340, University of South Alabama School of Medicine, Mobile, AL, 36688, USA. bfouty@jaguar1.usouthal.edu, Moss T, Solodushko V, Kraft M |
| المصدر: | The European respiratory journal [Eur Respir J] 2006 Jun; Vol. 27 (6), pp. 1160-7. Date of Electronic Publication: 2006 Mar 01. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: European Respiratory Society Country of Publication: England NLM ID: 8803460 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0903-1936 (Print) Linking ISSN: 09031936 NLM ISO Abbreviation: Eur Respir J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Sheffield, United Kingdom : European Respiratory Society Original Publication: Copenhagen : Published jointly by the Society and Munksgaard, 1988- |
| مواضيع طبية MeSH: | Anti-Inflammatory Agents/*pharmacology , Asthma/*pathology , Bronchial Hyperreactivity/*pathology , Dexamethasone/*pharmacology , Fibroblasts/*drug effects , G1 Phase/*drug effects , Muscle, Smooth/*drug effects , S Phase/*drug effects, Adult ; Biopsy ; Bronchi/drug effects ; Bronchi/pathology ; Bronchoscopy ; Cell Division/drug effects ; Cells, Cultured ; Female ; Fibroblasts/pathology ; Genes, Tumor Suppressor/drug effects ; Humans ; In Vitro Techniques ; Lung/drug effects ; Lung/pathology ; Male ; Muscle, Smooth/pathology ; Phosphorylation/drug effects ; Retinoblastoma Protein/drug effects ; Stimulation, Chemical |
| مستخلص: | Corticosteroids are the first line of therapy for asthma. Whether they alter the progression of airway remodelling in asthma is, as yet, unknown. To determine whether corticosteroids could alter the fibroblast cell cycle the current authors studied the effect of dexamethasone on cultured airway fibroblasts obtained from nine mild-to-moderate, steroid-naïve asthmatics (forced expiratory volume in one second 78+/-4% predicted), and seven normal controls. Fibroblasts were cultured from endobronchial biopsies obtained via bronchoscopy. Cells were exposed to dexamethasone (10(-9)-10(-7) M) and studied at 72 h to determine differences in progression through the cell cycle. In asthmatic fibroblasts, dexamethasone, at concentrations of 10(-8)M and 10(-7)M, nearly doubled the number of cells in the S phase (17.8+/-3.0% and 18.4+/-3.1%, respectively) compared with untreated fibroblasts (10.3+/-1.4%). There was no significant effect in normal control fibroblasts. Dexamethasone induced hyperphosphorylation of the tumour suppressor, retinoblastoma (RB) in asthmatic fibroblasts; fibroblasts from normal controls had significantly less hyperphosphorylation of RB. No difference in protein expression of the CCAAT/enhancer binding protein alpha between the two groups was detected. This study suggests that dexamethasone can stimulate G1-S phase cell cycle transition in human airway fibroblasts obtained from asthmatics. Whether this leads to enhanced airway remodelling in some individuals remains to be determined. |
| معلومات مُعتمدة: | HL64619 United States HL NHLBI NIH HHS; R01 HL070273 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Retinoblastoma Protein) 7S5I7G3JQL (Dexamethasone) |
| تواريخ الأحداث: | Date Created: 20060303 Date Completed: 20061109 Latest Revision: 20141120 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1183/09031936.06.00078605 |
| PMID: | 16510457 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0903-1936 |
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| DOI: | 10.1183/09031936.06.00078605 |