دورية أكاديمية
Ultrasound-targeted microbubble destruction improves the low density lipoprotein receptor gene expression in HepG2 cells.
| العنوان: | Ultrasound-targeted microbubble destruction improves the low density lipoprotein receptor gene expression in HepG2 cells. |
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| المؤلفون: | Guo DP; Institute of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China., Li XY, Sun P, Tang YB, Chen XY, Chen Q, Fan LM, Zang B, Shao LZ, Li XR |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2006 May 05; Vol. 343 (2), pp. 470-4. Date of Electronic Publication: 2006 Mar 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Sonication*, DNA/*administration & dosage , Gene Targeting/*methods , Hepatoblastoma/*genetics , Hepatoblastoma/*metabolism , Receptors, LDL/*metabolism , Transfection/*methods, Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Humans ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism ; Receptors, LDL/genetics |
| مستخلص: | Ultrasound-targeted microbubble destruction had been employed in gene delivery and promised great potential. Liver has unique features that make it attractive for gene therapy. However, it poses formidable obstacles to hepatocyte-specific gene delivery. This study was designed to test the efficiency of therapeutic gene transfer and expression mediated by ultrasound/microbubble strategy in HepG2 cell line. Air-filled albumin microbubbles were prepared and mixed with plasmid DNA encoding low density lipoprotein receptor (LDLR) and green fluorescent protein. The mixture of the DNA and microbubbles was administer to cultured HepG2 cells under variable ultrasound conditions. Transfection rate of the transferred gene and cell viability were assessed by FACS analysis, confocal laser scanning microscopy, Western blot analysis and Trypan blue staining. The result demonstrated that microbubbles with ultrasound irradiation can significantly elevate exogenous LDLR gene expression and the expressed LDLRs were functional and active to uptake their ligands. We conclude that ultrasound-targeted microbubble destruction has the potential to promote safe and efficient LDLR gene transfer into hepatocytes. With further refinement, it may represent an effective nonviral avenue of gene therapy for liver-involved genetic diseases. |
| المشرفين على المادة: | 0 (Receptors, LDL) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20060321 Date Completed: 20060516 Latest Revision: 20061115 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.bbrc.2006.02.179 |
| PMID: | 16546122 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-291X |
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| DOI: | 10.1016/j.bbrc.2006.02.179 |