دورية أكاديمية
Influence of paraformaldehyde and EDAC fixation on the demonstrability of hormones (histamine, endorphin, triiodothyronine) in rat immune cells: an immunocytochemical comparative analysis.
| العنوان: | Influence of paraformaldehyde and EDAC fixation on the demonstrability of hormones (histamine, endorphin, triiodothyronine) in rat immune cells: an immunocytochemical comparative analysis. |
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| المؤلفون: | Csaba G; Department of Genetics, Cell and Immunobiology, Semmelweis University, POB 370, 1445 Budapest, Hungary. csagyor@dgci.sote.hu, Kovács P, Pállinger E |
| المصدر: | Cell biology international [Cell Biol Int] 2006 May; Vol. 30 (5), pp. 412-5. Date of Electronic Publication: 2006 Mar 20. |
| نوع المنشور: | Comparative Study; Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Chichester Country of Publication: England NLM ID: 9307129 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1065-6995 (Print) Linking ISSN: 10656995 NLM ISO Abbreviation: Cell Biol Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2013- : John Wiley & Sons Chichester : Original Publication: London, UK : Published for the International Federation for Cell Biology by Academic Press, c1993- |
| مواضيع طبية MeSH: | Endorphins/*analysis , Ethyldimethylaminopropyl Carbodiimide/*analogs & derivatives , Formaldehyde/*pharmacology , Histamine/*analysis , Immunohistochemistry/*methods , Polymers/*pharmacology , Triiodothyronine/*analysis, Animals ; Ethyldimethylaminopropyl Carbodiimide/pharmacology ; Female ; Fixatives/pharmacology ; Granulocytes/cytology ; Lymphocytes/cytology ; Macrophages/cytology ; Male ; Mast Cells/cytology ; Monocytes/cytology ; Rats ; Rats, Wistar ; Sex Characteristics |
| مستخلص: | The amount and localization of three hormones (histamine, endorphin and triiodothyronine [T(3)]) was measured in male and female rat peritoneal cells (lymphocytes, mast cells, monocyte-macrophage-granulocyte group [mo-gran]) using flow cytometry as well as confocal microscopy after paraformaldehyde (PFA) or EDAC fixation. In the EDAC fixed lymphocytes and mo-gran of female animals two-magnitude higher levels of histamine were measured after EDAC fixation and one magitude higher in mast cells. The amount of T(3) was almost four-fold in lymphocytes and 2.5-4-fold in mast cells and mo-gran. Endorphin content was not altered by the type of fixation. In each cell type in males one magnitude higher levels of histamine and T(3) were measured after EDAC fixation and a small, but significant, elevation of endorphin. Confocal microscopy supports the quantitative data. The results show that (1) the fixation with the crosslinking molecule, EDAC, is more suitable for immunocytochemical studies of amino-acid type hormones in immune cells, (2) more histamine and T(3) are present in the immune cells than it was supposed previously when studying PFA-fixed preparations, (3) the estimation of the amount of peptide hormones seems to be accurate after PFA fixation, (4) there is a quantitative difference comparing the results of PFA and EDAC fixation between males and females. |
| المشرفين على المادة: | 0 (Endorphins) 0 (Fixatives) 0 (Polymers) 06LU7C9H1V (Triiodothyronine) 1HG84L3525 (Formaldehyde) 73U5123RNT (1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide) 820484N8I3 (Histamine) RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide) Y19UC83H8E (paraform) |
| تواريخ الأحداث: | Date Created: 20060321 Date Completed: 20060803 Latest Revision: 20191210 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1016/j.cellbi.2006.01.005 |
| PMID: | 16546412 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1065-6995 |
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| DOI: | 10.1016/j.cellbi.2006.01.005 |