دورية أكاديمية
The sulfonated osmolyte N-methyltaurine is dissimilated by Alcaligenes faecalis and by Paracoccus versutus with release of methylamine.
| العنوان: | The sulfonated osmolyte N-methyltaurine is dissimilated by Alcaligenes faecalis and by Paracoccus versutus with release of methylamine. |
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| المؤلفون: | Weinitschke S; Department of Biology, University of Konstanz, D-78457 Konstanz, Germany., Denger K; Department of Biology, University of Konstanz, D-78457 Konstanz, Germany., Smits THM; Department of Biology, University of Konstanz, D-78457 Konstanz, Germany., Hollemeyer K; Institute of Biochemical Engineering, Saarland University, Box 50 11 50, D-66041 Saarbrücken, Germany., Cook AM; Department of Biology, University of Konstanz, D-78457 Konstanz, Germany. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2006 Apr; Vol. 152 (Pt 4), pp. 1179-1186. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print Cited Medium: Print ISSN: 1350-0872 (Print) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Alcaligenes faecalis/*metabolism , Methylamines/*metabolism , Paracoccus/*metabolism , Taurine/*analogs & derivatives , Taurine/*metabolism, Acetyltransferases/analysis ; Alcaligenes faecalis/classification ; Alcaligenes faecalis/growth & development ; Alcaligenes faecalis/isolation & purification ; Bacterial Proteins/genetics ; Carbon/metabolism ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Mass Spectrometry ; Molecular Sequence Data ; Multigene Family ; Nitrogen/metabolism ; Oxidoreductases/analysis ; Oxidoreductases Acting on CH-NH Group Donors/analysis ; Paracoccus/classification ; Paracoccus/growth & development ; Paracoccus/isolation & purification ; Sequence Analysis, DNA ; Sewage/microbiology ; Sulfite Dehydrogenase/analysis ; Water Microbiology |
| مستخلص: | Selective enrichments yielded bacterial cultures able to utilize the osmolyte N-methyltaurine as sole source of carbon and energy or as sole source of fixed nitrogen for aerobic growth. Strain MT1, which degraded N-methyltaurine as a sole source of carbon concomitantly with growth, was identified as a strain of Alcaligenes faecalis. Stoichiometric amounts of methylamine, whose identity was confirmed by matrix-assisted, laser-desorption ionization time-of-flight mass spectrometry, and of sulfate were released during growth. Inducible N-methyltaurine dehydrogenase, sulfoacetaldehyde acetyltransferase (Xsc) and a sulfite dehydrogenase could be detected. Taurine dehydrogenase was also present and it was hypothesized that taurine dehydrogenase has a substrate range that includes N-methyltaurine. Partial sequences of a tauY-like gene (encoding the putative large component of taurine dehydrogenase) and an xsc gene were obtained by PCR with degenerate primers. Strain N-MT utilized N-methyltaurine as a sole source of fixed nitrogen for growth and could also utilize the compound as sole source of carbon. This bacterium was identified as a strain of Paracoccus versutus. This organism also expressed inducible (N-methyl)taurine dehydrogenase, Xsc and a sulfite dehydrogenase. The presence of a gene cluster with high identity to a larger cluster from Paracoccus pantotrophus NKNCYSA, which is now known to dissimilate N-methyltaurine via Xsc, allowed most of the overall pathway, including transport and excretion, to be defined. N-Methyltaurine is thus another compound whose catabolism is channelled directly through sulfoacetaldehyde. |
| سلسلة جزيئية: | GENBANK AM048879; AM048880; AM048881; AM048882; AM087667; AM087668 |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (DNA, Bacterial) 0 (Methylamines) 0 (Sewage) 1EQV5MLY3D (Taurine) 7440-44-0 (Carbon) BSF23SJ79E (methylamine) EC 1.- (Oxidoreductases) EC 1.4.99.2 (taurine dehydrogenase) EC 1.5.- (Oxidoreductases Acting on CH-NH Group Donors) EC 1.8.2.1 (Sulfite Dehydrogenase) EC 2.3.1.- (Acetyltransferases) N762921K75 (Nitrogen) |
| تواريخ الأحداث: | Date Created: 20060322 Date Completed: 20060721 Latest Revision: 20201109 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/mic.0.28622-0 |
| PMID: | 16549680 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1350-0872 |
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| DOI: | 10.1099/mic.0.28622-0 |