دورية أكاديمية
Proteomics approaches to study the redox state of cysteine-containing proteins.
| العنوان: | Proteomics approaches to study the redox state of cysteine-containing proteins. |
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| المؤلفون: | Camerini S; Unita di Spettrometria di Massa, DIBIT, Istituto Scientifico San Raffaele, Milan, Italy., Polci ML, Bachi A |
| المصدر: | Annali dell'Istituto superiore di sanita [Ann Ist Super Sanita] 2005; Vol. 41 (4), pp. 451-7. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Istituto superiore di sanita Country of Publication: Italy NLM ID: 7502520 Publication Model: Print Cited Medium: Print ISSN: 0021-2571 (Print) Linking ISSN: 00212571 NLM ISO Abbreviation: Ann Ist Super Sanita Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Roma : Istituto superiore di sanità Original Publication: Roma. |
| مواضيع طبية MeSH: | Cysteine/*chemistry , Proteins/*chemistry , Proteomics/*methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods, Animals ; Biotinylation ; Cattle ; Ethylmaleimide/pharmacology ; HeLa Cells ; Humans ; Hydrogen-Ion Concentration ; Nitroso Compounds/chemistry ; Ovalbumin/chemistry ; Oxidation-Reduction ; Ribonucleases/chemistry ; Serum Albumin, Bovine/chemistry ; Sulfhydryl Reagents/pharmacology |
| مستخلص: | All the proteins synthesized in a cell undergo several post-translational modifications that are essential in their functional regulation. Among these, the change of the redox state of Cysteine residues is assuming a great interest: this modification in fact, represents a very dynamic and regulated balance. There are several reversible oxidative events that can occur and that are difficult to detect. In this work we describe a methodology useful to recognize and to select Cysteines containing proteins on the basis of their redox state. The strategy is based on the selective labeling of the interested proteins and allows their visualization by Western Blot, enrichment by affinity chromatography and finally the identification of the protein and of the modified Cysteine residues by mass spectrometry. This methodology can be used in proteomic studies to recognize redox-sensitive Cysteine containing proteins and nitric oxide targets. |
| المشرفين على المادة: | 0 (Nitroso Compounds) 0 (Proteins) 0 (Sulfhydryl Reagents) 27432CM55Q (Serum Albumin, Bovine) 9006-59-1 (Ovalbumin) EC 3.1.- (Ribonucleases) K848JZ4886 (Cysteine) O3C74ACM9V (Ethylmaleimide) |
| تواريخ الأحداث: | Date Created: 20060330 Date Completed: 20060811 Latest Revision: 20171116 |
| رمز التحديث: | 20240628 |
| PMID: | 16569913 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0021-2571 |
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