دورية أكاديمية
Calcium ionophore-induced de-encryption of tissue factor in monocytes is associated with extensive cell death.
| العنوان: | Calcium ionophore-induced de-encryption of tissue factor in monocytes is associated with extensive cell death. |
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| المؤلفون: | Henriksson CE; The R&D Group, Department of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway. carola.henriksson@medisin.uio.no, Klingenberg O, Hellum M, Landsverk KS, Joø GB, Westvik AB, Kierulf P |
| المصدر: | Thrombosis research [Thromb Res] 2007; Vol. 119 (5), pp. 621-30. Date of Electronic Publication: 2006 Jul 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: United States NLM ID: 0326377 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0049-3848 (Print) Linking ISSN: 00493848 NLM ISO Abbreviation: Thromb Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Elmsford, N. Y., Pergamon Press. |
| مواضيع طبية MeSH: | Calcium/*metabolism , Ionophores/*pharmacology , Monocytes/*drug effects , Thromboplastin/*drug effects, Blood Coagulation/drug effects ; Cell Death/drug effects ; Cell Death/physiology ; Dose-Response Relationship, Drug ; Factor Xa/analysis ; Factor Xa/biosynthesis ; Flow Cytometry ; Humans ; Monocytes/metabolism ; Thromboplastin/metabolism |
| مستخلص: | Introduction: Cell surface tissue factor (TF) is normally encrypted, but can be activated by various cellular perturbations. Exposure of TF bearing cells to calcium ionophore has been reported to increase TF activity, de-encrypt TF, by phosphatidylserine (PS)-dependent and -independent mechanisms. Our aim has been to examine at the single cell level, if increased cell surface PS coincided with increased cell surface TF antigen, and cell death (necrosis, 7-AAD-intercalation), and relate this to monocyte- and microparticle (MP)-associated procoagulant activity. Materials and Methods: We exposed lipopolysaccharide-stimulated, human, elutriation-purified, cryopreserved TF bearing monocytes to increasing concentrations of calcium ionophore (A23187) and measured procoagulant activity in cells and supernatants. These measurements were compared with quantification of cell surface TF and PS (Annexin V) and of cell necrosis (7-AAD) by flow cytometry, and complemented by confocal microscopy. Results: We observed that calcium ionophore increased cellular and MP-associated TF activity, but not cell surface TF antigen. The discrepancy between TF activity and TF antigen coincided with a dose-dependent increase in the number of cells expressing PS. These cells were to a large extent necrotic and many of them also expressed TF. Conclusions: We suggest such TF positive dying cells to contribute to the discordance between TF activity and TF expression. Calcium ionophore also increased MP-associated TF activity and release of MPs may be a way to disseminate procoagulant activity. Our findings emphasize the importance of adequately assessing cell death and taking into consideration its possible role in experiments with calcium ionophore. |
| المشرفين على المادة: | 0 (Ionophores) 9035-58-9 (Thromboplastin) EC 3.4.21.6 (Factor Xa) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20060718 Date Completed: 20071211 Latest Revision: 20220224 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.thromres.2006.05.012 |
| PMID: | 16844202 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 0049-3848 |
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| DOI: | 10.1016/j.thromres.2006.05.012 |