دورية أكاديمية
Beta-adrenoceptor distribution in murine lymphoid cell lines.
| العنوان: | Beta-adrenoceptor distribution in murine lymphoid cell lines. |
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| المؤلفون: | Cremaschi GA; Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Fisher P, Boege F |
| المصدر: | Immunopharmacology [Immunopharmacology] 1991 Nov-Dec; Vol. 22 (3), pp. 195-206. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7902474 Publication Model: Print Cited Medium: Print ISSN: 0162-3109 (Print) Linking ISSN: 01623109 NLM ISO Abbreviation: Immunopharmacology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier/North-Holland Original Publication: New York, Elsevier/North-Holland. |
| مواضيع طبية MeSH: | Lymphocytes/*chemistry , Lymphoma/*metabolism , Receptors, Adrenergic, beta/*analysis, Adenylyl Cyclases/analysis ; Adrenergic beta-Agonists/pharmacology ; Alprostadil/pharmacology ; Animals ; Cell Line ; Cyclic AMP/biosynthesis ; Iodocyanopindolol ; Mice ; Pindolol/analogs & derivatives ; Pindolol/metabolism ; Receptors, Adrenergic, beta/physiology |
| مستخلص: | beta-Adrenergic receptors (R) on several tumor lymphoid cell lines were characterized both directly by beta radioligand binding of 125iodo-cyanopindolol (125I-CYP) to intact cells and membranes, and functionally by assessing hormone-dependent changes in cyclic 3',5' adenosine monophosphate (cAMP) levels on intact cells and measuring adenylate cyclase (a.c.) activity on membranes. Only two lymphoid cell types, BW 5147 (a T cell derived lymphoma cell line) and TIB 221 (a B cell derived line) displayed significant amounts of beta-adrenergic R by 125I-CYP specific binding. Despite this, no stimulation of the a.c. activity was found in the presence of beta-adrenergic agonists in these cells in comparison with native lymphocytes or cells of the well-known S49 cell line used as a positive control. beta-Adrenoceptor specific uncoupling was confirmed by aluminum tetrafluoride (AlFl4) direct activation of the a.c. system in the beta R-bearing cell membranes and by an increase in cAMP production induced by PGE1, another hormone that activates the a.c. Structural characterization of beta-adrenoceptors by photoaffinity-labeling demonstrates that uncoupling was not due to a structural alteration of the beta-adrenergic R expressed in these lymphoma cell lines, as these R gave similar results as native or S49 cells. It can be concluded that functional beta-adrenoceptors are absent in these lymphoma cells. The possible implication of alternative transmission pathways and original neuroendocrine control in tumor lymphoid cells is discussed. |
| المشرفين على المادة: | 0 (Adrenergic beta-Agonists) 0 (Receptors, Adrenergic, beta) 83498-72-0 (Iodocyanopindolol) BJ4HF6IU1D (Pindolol) E0399OZS9N (Cyclic AMP) EC 4.6.1.1 (Adenylyl Cyclases) F5TD010360 (Alprostadil) |
| تواريخ الأحداث: | Date Created: 19911101 Date Completed: 19920304 Latest Revision: 20190918 |
| رمز التحديث: | 20221208 |
| DOI: | 10.1016/0162-3109(91)90044-y |
| PMID: | 1685489 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0162-3109 |
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| DOI: | 10.1016/0162-3109(91)90044-y |