دورية أكاديمية
أعرض في EDS

Activation of Wnt signaling in the intestinal mucosa of Apc +/min mice does not cause overexpression of the receptor tyrosine kinase Met.

التفاصيل البيبلوغرافية
العنوان: Activation of Wnt signaling in the intestinal mucosa of Apc +/min mice does not cause overexpression of the receptor tyrosine kinase Met.
المؤلفون: Boon EM; Department of Pathology, Academic Medical Center, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands., Pouwels W, Redeker S, Joosten SP, Hamann J, van der Neut R, Pals ST
المصدر: Cancer science [Cancer Sci] 2006 Aug; Vol. 97 (8), pp. 710-5.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Publishing on behalf of the Japanese Cancer Association Country of Publication: England NLM ID: 101168776 Publication Model: Print Cited Medium: Print ISSN: 1347-9032 (Print) Linking ISSN: 13479032 NLM ISO Abbreviation: Cancer Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2005- : Oxford : Wiley Publishing on behalf of the Japanese Cancer Association
Original Publication: Tokyo : Japanese Cancer Association, c2003-
مواضيع طبية MeSH: Adenomatous Polyposis Coli Protein/*genetics , Intestinal Mucosa/*enzymology , Intestinal Neoplasms/*enzymology , Proto-Oncogene Proteins c-met/*metabolism , Wnt Proteins/*metabolism, Animals ; Antibodies, Monoclonal ; Cricetinae ; Humans ; Immunochemistry ; Intestinal Mucosa/chemistry ; Intestinal Mucosa/embryology ; Intestinal Neoplasms/chemistry ; Mice ; Mice, Mutant Strains ; Microdissection ; Proto-Oncogene Proteins c-met/analysis ; Proto-Oncogene Proteins c-met/genetics ; RNA, Messenger/analysis ; RNA, Messenger/metabolism ; Receptor Protein-Tyrosine Kinases/analysis ; Receptor Protein-Tyrosine Kinases/genetics ; Receptor Protein-Tyrosine Kinases/metabolism
مستخلص: The receptor tyrosine kinase MET is overexpressed in human colorectal adenomas and carcinomas, suggesting an instrumental role for MET signaling in the onset and progression of colorectal cancer. To corroborate this role, animal models are needed. To study the expression of Met in the normal and neoplastic mouse intestine, we generated an Armenian hamster monoclonal antibody against mouse Met. By using this antibody in immunohistochemical studies, we observed strong Met expression in fetal mouse intestinal epithelial cells. In contrast, in the intestines of adult mice, Met expression was very low whereas the protein was undetectable on the neoplastic epithelium of intestinal adenomas in Apc+/min mice. By immunoblotting, we were also unable to detect Met in intestinal adenomas, whereas Met mRNA levels in microdissected adenomas were very low. The absence of detectable Met protein expression in adenomas of Apc+/min mice contrasts sharply with the vast overexpression of the protein in adenomas of humans with familial adenomatous polyposis or sporadic colorectal carcinomas. Our results imply that deregulation of Wnt signaling in mouse--unlike in human--intestinal epithelium does not result in Met overexpression. Our findings thus reveal important interspecies differences in the regulation of Met expression during intestinal tumorigenesis.
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المشرفين على المادة: 0 (Adenomatous Polyposis Coli Protein)
0 (Antibodies, Monoclonal)
0 (RNA, Messenger)
0 (Wnt Proteins)
EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
EC 2.7.10.1 (RON protein)
EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
تواريخ الأحداث: Date Created: 20060726 Date Completed: 20060921 Latest Revision: 20240609
رمز التحديث: 20240609
مُعرف محوري في PubMed: PMC11159999
DOI: 10.1111/j.1349-7006.2006.00238.x
PMID: 16863504
قاعدة البيانات: MEDLINE
الوصف
تدمد:1347-9032
DOI:10.1111/j.1349-7006.2006.00238.x