دورية أكاديمية
Effect of chronic prenatal morphine treatment of mu-opioid receptor-regulated adenylate cyclase activity and neurotransmitter release in rat brain slices.
| العنوان: | Effect of chronic prenatal morphine treatment of mu-opioid receptor-regulated adenylate cyclase activity and neurotransmitter release in rat brain slices. |
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| المؤلفون: | De Vries TJ; Department of Pharmacology, Medical Faculty, Free University, Amsterdam, The Netherlands., Van Vliet BJ, Hogenboom F, Wardeh G, Van der Laan JW, Mulder AH, Schoffelmeer AN |
| المصدر: | European journal of pharmacology [Eur J Pharmacol] 1991 Oct 14; Vol. 208 (2), pp. 97-104. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 1254354 Publication Model: Print Cited Medium: Print ISSN: 0014-2999 (Print) Linking ISSN: 00142999 NLM ISO Abbreviation: Eur J Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Amsterdam : Elsevier Science Original Publication: Amsterdam, North Holland Pub. Co. |
| مواضيع طبية MeSH: | Adenylyl Cyclases/*drug effects , Brain/*drug effects , Morphine/*pharmacology , Neurotransmitter Agents/*metabolism , Receptors, Opioid/*physiology, Adaptation, Physiological ; Adenylyl Cyclases/metabolism ; Animals ; Brain/embryology ; Brain/enzymology ; Female ; Fetus/drug effects ; Fetus/physiology ; Norepinephrine/metabolism ; Pregnancy ; Prenatal Exposure Delayed Effects ; Rats ; Rats, Inbred Strains ; Receptors, Opioid/drug effects ; Receptors, Opioid, mu ; Synaptic Transmission/drug effects |
| مستخلص: | Timed-pregnant rats received a semisynthetic diet with or without morphine (0.5-1 mg/g) for 2 weeks. After 21 days of gestation the morphine-dependent dams were decapitated and the foetal brains were dissected. Chronic morphine administration caused a profound increase of adenylate cyclase activity stimulated by postsynaptic D1 dopamine receptors in striatal slices. The relative inhibitory effect of [D-Ala2,MePhe4,Gly-ol5]enkephalin (DAGO) on D1-stimulated cyclic AMP (cAMP) production was unaffected. In contrast, cAMP production induced via direct activation of the catalytic unit of adenylate cyclase with forskolin was not changed upon long-term morphine treatment, although DAGO strongly inhibited the effect of forskolin. The electrically evoked release of [3H]noradrenaline (NA) from superfused neocortical slices was strongly enhanced upon morphine treatment, whereas release induced by the calcium ionophore A23187, bypassing voltage-sensitive calcium channels, was unchanged. Again, the inhibitory effect of the mu receptor agonist DAGO was unaffected in neocortical slices from morphine-treated rats. It is suggested that tolerance to morphine may be caused by the fact that the opiate is acting against up-regulated signal transduction mechanisms, rather than by desensitization of central mu-opioid receptors. The pre- and postsynaptic changes may include an enhanced expression and/or biochemical modification of D1 receptors, Gs proteins and calcium channels in central neurons on which mu-opioid receptors are present. At the same time, these adaptive changes may underlie morphine withdrawal phenomena. |
| المشرفين على المادة: | 0 (Neurotransmitter Agents) 0 (Receptors, Opioid) 0 (Receptors, Opioid, mu) 76I7G6D29C (Morphine) EC 4.6.1.1 (Adenylyl Cyclases) X4W3ENH1CV (Norepinephrine) |
| تواريخ الأحداث: | Date Created: 19911014 Date Completed: 19920421 Latest Revision: 20190624 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1016/0922-4106(91)90059-q |
| PMID: | 1686867 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-2999 |
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| DOI: | 10.1016/0922-4106(91)90059-q |