دورية أكاديمية
The blue-light receptor YtvA acts in the environmental stress signaling pathway of Bacillus subtilis.
| العنوان: | The blue-light receptor YtvA acts in the environmental stress signaling pathway of Bacillus subtilis. |
|---|---|
| المؤلفون: | Gaidenko TA; Department of Food Science and Technology University of California, Davis, CA 95616, USA., Kim TJ, Weigel AL, Brody MS, Price CW |
| المصدر: | Journal of bacteriology [J Bacteriol] 2006 Sep; Vol. 188 (17), pp. 6387-95. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print Cited Medium: Print ISSN: 0021-9193 (Print) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Society for Microbiology |
| مواضيع طبية MeSH: | Gene Expression Regulation, Bacterial*, Bacillus subtilis/*physiology , Bacterial Proteins/*genetics , Bacterial Proteins/*metabolism , Flavoproteins/*genetics , Flavoproteins/*metabolism, Bacillus subtilis/genetics ; Bacterial Proteins/chemistry ; Cysteine ; Disulfides ; Flavin Mononucleotide/metabolism ; Flavoproteins/chemistry ; Heat-Shock Proteins/genetics ; Heat-Shock Proteins/metabolism ; Protein Structure, Tertiary ; Sigma Factor/genetics ; Sigma Factor/metabolism ; Signal Transduction |
| مستخلص: | The general stress response of the bacterium Bacillus subtilis is regulated by a partner-switching mechanism in which serine and threonine phosphorylation controls protein interactions in the stress-signaling pathway. The environmental branch of this pathway contains a family of five paralogous proteins that function as negative regulators. Here we present genetic evidence that a sixth paralog, YtvA, acts as a positive regulator in the same environmental signaling branch. We also present biochemical evidence that YtvA and at least three of the negative regulators can be isolated from cell extracts in a large environmental signaling complex. YtvA differs from these associated negative regulators by its flavin mononucleotide (FMN)-containing light-oxygen-voltage domain. Others have shown that this domain has the photochemistry expected for a blue-light sensor, with the covalent linkage of the FMN chromophore to cysteine 62 composing a critical part of the photocycle. Consistent with the view that light intensity modifies the output of the environmental signaling pathway, we found that cysteine 62 is required for YtvA to exert its positive regulatory role in the absence of other stress. Transcriptional analysis of the ytvA structural gene indicated that it provides the entry point for at least one additional environmental input, mediated by the Spx global regulator of disulfide stress. These results support a model in which the large signaling complex serves to integrate multiple environmental signals in order to modulate the general stress response. |
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| معلومات مُعتمدة: | R01 GM042077 United States GM NIGMS NIH HHS; GM42077 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Disulfides) 0 (Flavoproteins) 0 (Heat-Shock Proteins) 0 (SigB protein, Bacteria) 0 (Sigma Factor) 7N464URE7E (Flavin Mononucleotide) K848JZ4886 (Cysteine) |
| تواريخ الأحداث: | Date Created: 20060823 Date Completed: 20061002 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC1595380 |
| DOI: | 10.1128/JB.00691-06 |
| PMID: | 16923906 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9193 |
|---|---|
| DOI: | 10.1128/JB.00691-06 |