دورية أكاديمية
Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare.
| العنوان: | Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare. |
|---|---|
| المؤلفون: | Fiala SM; Reprolab-Departamento de Medicina Animal, Faculdade de Veterinária, UFRGS, Caixa Postal 15039, 91501-970 Porto Alegre, RS, Brazil., Pimentel CA, Mattos AL, Gregory RM, Mattos RC |
| المصدر: | Theriogenology [Theriogenology] 2007 Feb; Vol. 67 (3), pp. 556-62. Date of Electronic Publication: 2006 Oct 10. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0093-691X (Print) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| مواضيع طبية MeSH: | Horse Diseases/*physiopathology , Inflammation/*veterinary , Insemination, Artificial/*veterinary , Sperm Count/*veterinary , Sperm Transport/*physiology , Uterine Diseases/*veterinary, Animals ; Cell Count/veterinary ; Female ; Horses ; Inflammation/physiopathology ; Male ; Milk/physiology ; Neutrophils/cytology ; Time Factors ; Uterine Diseases/physiopathology |
| مستخلص: | Our objective was to determine whether the concentration of cooled sperm inseminated influenced sperm transport and intensity of the uterine inflammatory reaction 2, 4 and 24h after insemination. Experimental subjects were 189 estrous mares with a dominant follicle > or =35 mm in diameter and no bacterial growth or neutrophils detected in uterine smears. Each mare was randomly assigned to receive one of the following intrauterine treatments (volume, 20 mL): insemination with 5x10(6) mL(-1) or 25x10(6) mL(-1) or 50x10(6) mL(-1) sperm diluted in 3 mL seminal plasma (SP) and 17 mL skim milk; seminal plasma or skim milk extender. Mares in a control group received no intrauterine treatment. Mares were slaughtered 2, 4 or 24h after insemination or infusion. Oviducts were separated from the uterus, and uterus and oviducts were then flushed with phosphate-buffered saline (PBS). After flushing, an endometrial sample was collected for further histopathological examination. The grade of uterine fibrosis and the amount of neutrophils in the stratum compactum were evaluated. A sample of each tubal flushing was examined for sperm count, and a sample of each uterine flushing was examined for PMN count. It was concluded that compounds in the insemination dose provoked a uterine inflammatory response, which was more rapid and intense as sperm concentration increased. In contrast, sperm transport through 4h after insemination was not influenced by sperm concentration. |
| تواريخ الأحداث: | Date Created: 20061013 Date Completed: 20080903 Latest Revision: 20070108 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.theriogenology.2006.09.005 |
| PMID: | 17034841 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0093-691X |
|---|---|
| DOI: | 10.1016/j.theriogenology.2006.09.005 |