دورية أكاديمية
Arachidonic acid potentiates exocytosis and allows neuronal SNARE complex to interact with Munc18a.
| العنوان: | Arachidonic acid potentiates exocytosis and allows neuronal SNARE complex to interact with Munc18a. |
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| المؤلفون: | Latham CF; Molecular Dynamics of Synaptic Function Laboratory, School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia., Osborne SL, Cryle MJ, Meunier FA |
| المصدر: | Journal of neurochemistry [J Neurochem] 2007 Mar; Vol. 100 (6), pp. 1543-54. Date of Electronic Publication: 2006 Dec 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley on behalf of the International Society for Neurochemistry Country of Publication: England NLM ID: 2985190R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0022-3042 (Print) Linking ISSN: 00223042 NLM ISO Abbreviation: J Neurochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2001- : Oxford, UK : Wiley on behalf of the International Society for Neurochemistry Original Publication: New York : Raven Press |
| مواضيع طبية MeSH: | Arachidonic Acid/*pharmacology , Exocytosis/*drug effects , Munc18 Proteins/*metabolism , SNARE Proteins/*metabolism, Adenosine Triphosphate/pharmacology ; Animals ; Brain/cytology ; Brain/drug effects ; Calcium/pharmacology ; Catecholamines/metabolism ; Cattle ; Chromaffin Cells ; Dose-Response Relationship, Drug ; Drug Interactions ; Neurons/drug effects ; Neurons/metabolism ; Permeability/drug effects ; Protein Binding/drug effects |
| مستخلص: | Neuronal communication relies on the fusion of neurotransmitter-containing vesicles with the neuronal plasma membrane. Recent genetic studies have highlighted the critical role played by polyunsaturated fatty acids in neurotransmission, however, there is little information available about which fatty acids act on exocytosis and, more importantly, by what mechanism. We have used permeabilized chromaffin cells to screen various fatty acids of the n-3 and n-6 series for their acute effects on exocytosis. We have demonstrated that an n-6 series polyunsaturated fatty acid, arachidonic acid, potentiates secretion from intact neurosecretory cells regardless of the secretagogue used. We have shown that arachidonic acid dose dependently increases soluble NSF attachment protein receptor complex formation in chromaffin cells and bovine cortical brain extracts and that a non-hydrolysable analogue of arachidonic acid causes a similar increase in SNARE complex formation. This prompted us to examine the effect of arachidonic acid on SNARE protein interactions with Munc18a, a protein known to prevent Syntaxin1a engagement into the SNARE complex in vitro. In the presence of arachidonic acid, we show that Munc18a can interact with the neuronal SNARE complex in a dose-dependent manner. We further demonstrate that arachidonic acid directly interacts with Syntaxin1a. |
| المشرفين على المادة: | 0 (Catecholamines) 0 (Munc18 Proteins) 0 (SNARE Proteins) 27YG812J1I (Arachidonic Acid) 8L70Q75FXE (Adenosine Triphosphate) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20061222 Date Completed: 20070501 Latest Revision: 20131121 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/j.1471-4159.2006.04286.x |
| PMID: | 17181552 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0022-3042 |
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| DOI: | 10.1111/j.1471-4159.2006.04286.x |