دورية أكاديمية
A comparative evaluation between real time Roche COBas TAQMAN 48 HCV and bDNA Bayer Versant HCV 3.0.
| العنوان: | A comparative evaluation between real time Roche COBas TAQMAN 48 HCV and bDNA Bayer Versant HCV 3.0. |
|---|---|
| المؤلفون: | Giraldi C; UOC Microbiologia e Virologia, PO Annunziata AO Cosenza. gircri@virgilio.it, Noto A, Tenuta R, Greco F, Perugini D, Spadafora M, Bianco AM, Savino O, Natale A |
| المصدر: | The new microbiologica [New Microbiol] 2006 Oct; Vol. 29 (4), pp. 243-50. |
| نوع المنشور: | Comparative Study; Evaluation Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Edizioni Internazionali srl Country of Publication: Italy NLM ID: 9516291 Publication Model: Print Cited Medium: Print ISSN: 1121-7138 (Print) Linking ISSN: 11217138 NLM ISO Abbreviation: New Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2004- : Pavia, Italy : Edizioni Internazionali srl Original Publication: Pavia, Italy : L. Ponzio e figlio, 1993- |
| مواضيع طبية MeSH: | Branched DNA Signal Amplification Assay/*methods , Hepacivirus/*isolation & purification , Polymerase Chain Reaction/*methods , RNA, Viral/*blood, Genotype ; Hepacivirus/classification ; Hepacivirus/genetics ; Hepatitis C/virology ; Humans ; Taq Polymerase |
| مستخلص: | The HCV virus is a common human pathogen made of a single stranded RNA genome with 9600nt. This work compared two different commercial methods used for HCV viral load, the bDNA Bayer Versant HCV 3.0 and the RealTime Roche COBAS TaqMan 48 HCV. We compared the reproducibility and linearity of the two methods. Seventy-five plasma samples with genotypes 1 to 4, which represent the population (45% genotype 1; 24% genotype 2; 13% genotype 3; 18% genotype 4) were directly processed with the Versanto method based upon signal amplification; the same samples were first extracted (COBAS Ampliprep - TNAI) and then amplified using RealTime PCR (COBAS TaqMan 48). The results obtained indicate the same performance for both methods if they have genotype 1, but in samples with genotypes 2, 3 and 4 the RealTime PCR Roche method gave an underestimation in respect to the Bayer bDNA assay. |
| المشرفين على المادة: | 0 (RNA, Viral) EC 2.7.7.- (Taq Polymerase) |
| تواريخ الأحداث: | Date Created: 20070105 Date Completed: 20070201 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| PMID: | 17201090 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1121-7138 |
|---|