دورية أكاديمية
أعرض في EDS

Regulation of the human SOX9 promoter by Sp1 and CREB.

التفاصيل البيبلوغرافية
العنوان: Regulation of the human SOX9 promoter by Sp1 and CREB.
المؤلفون: Piera-Velazquez S; Department of Medicine, Division of Rheumatology, Thomas Jefferson University, Jefferson Medical College, 233 S. 10th Street, Room 509 BLSB, Philadelphia, PA 19107-5541, USA., Hawkins DF, Whitecavage MK, Colter DC, Stokes DG, Jimenez SA
المصدر: Experimental cell research [Exp Cell Res] 2007 Apr 01; Vol. 313 (6), pp. 1069-79. Date of Electronic Publication: 2007 Jan 08.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0014-4827 (Print) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE
أسماء مطبوعة: Publication: Orlando Fl : Academic Press
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Gene Expression Regulation* , Promoter Regions, Genetic*, Cyclic AMP Response Element-Binding Protein/*genetics , High Mobility Group Proteins/*genetics , Sp1 Transcription Factor/*genetics , Transcription Factors/*genetics, Animals ; Base Sequence ; Cell Line ; Chondrogenesis ; Cyclic AMP Response Element-Binding Protein/metabolism ; Deoxyribonuclease I/genetics ; Electrophoretic Mobility Shift Assay ; High Mobility Group Proteins/metabolism ; Humans ; Interleukin-1beta/pharmacology ; Mice ; Molecular Sequence Data ; Protein Binding ; Response Elements ; SOX9 Transcription Factor ; Transcription Factors/metabolism ; Transfection
مستخلص: The transcription factor SOX9 is essential for multiple steps during skeletal development, including mesenchymal cell chondrogenesis and endochondral bone formation. We recently reported that the human SOX9 proximal promoter region is regulated by the CCAAT-binding factor through two CCAAT boxes located within 100 bp of the transcriptional start site. Here we report that the human SOX9 proximal promoter is also regulated by the cyclic-AMP response element binding protein (CREB) and Sp1. We show by DNaseI protection and EMSA analysis that CREB and Sp1 interact with specific sites within the SOX9 proximal promoter region. By transient transfection analysis we also demonstrate that mutations of the CREB and Sp1 binding sites result in a profound reduction of SOX9 promoter activity. Chromatin immunoprecipitation (ChIP) assay demonstrated that both Sp1 and CREB interact with the SOX9 promoter in vivo. Finally, we demonstrate that IL-1beta treatment of chondrocytes isolated from human normal and osteoarthritic (OA) cartilage down-regulates SOX9 promoter activity, an effect accompanied by a reduction of Sp1 binding to the SOX9 proximal promoter.
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معلومات مُعتمدة: P01 AR039740 United States AR NIAMS NIH HHS; AR-39740 United States AR NIAMS NIH HHS
المشرفين على المادة: 0 (Cyclic AMP Response Element-Binding Protein)
0 (High Mobility Group Proteins)
0 (Interleukin-1beta)
0 (SOX9 Transcription Factor)
0 (SOX9 protein, human)
0 (Sox9 protein, mouse)
0 (Sp1 Transcription Factor)
0 (Transcription Factors)
EC 3.1.21.1 (Deoxyribonuclease I)
تواريخ الأحداث: Date Created: 20070210 Date Completed: 20070502 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC2118054
DOI: 10.1016/j.yexcr.2007.01.001
PMID: 17289023
قاعدة البيانات: MEDLINE
الوصف
تدمد:0014-4827
DOI:10.1016/j.yexcr.2007.01.001