دورية أكاديمية
أعرض في EDS

Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614.

التفاصيل البيبلوغرافية
العنوان: Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614.
المؤلفون: Mattes TE; Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, Iowa City, IA 52242, USA. tim-mattes@uiowa.edu, Coleman NV, Chuang AS, Rogers AJ, Spain JC, Gossett JM
المصدر: Archives of microbiology [Arch Microbiol] 2007 Mar; Vol. 187 (3), pp. 217-26. Date of Electronic Publication: 2006 Nov 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0302-8933 (Print) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin, New York, Springer-Verlag.
مواضيع طبية MeSH: Ethylenes/*metabolism , Nocardiaceae/*growth & development , Nocardiaceae/*metabolism , Vinyl Chloride/*metabolism, Adaptation, Physiological ; Biodegradation, Environmental ; Nocardiaceae/genetics ; Oxidative Stress/physiology
مستخلص: The extended lag period associated with vinyl chloride (VC) starvation in VC- and ethene-assimilating Nocardioides sp. strain JS614 was examined. The extended lag periods were variable (3-7 days), only associated with growth on VC or ethene, and were observed in VC- or ethene-grown cultures following 24 h carbon starvation and mid-exponential phase cultures grown on non-alkene carbon sources (e.g. acetate). Alkene monooxygenase (AkMO) and epoxyalkane:coenzyme M transferase (EaCoMT) are the initial enzymes of VC and ethene biodegradation in strain JS614. Reverse-transcription PCR confirmed that the AkMO gene etnC was expressed in response to epoxyethane, a metabolic intermediate of ethene biodegradation. Epoxyethane (0.5 mM) eliminated the extended lag period in both starved and mid-exponential phase cultures, suggesting that epoxyethane accumulation activates AkMO expression in strain JS614. AkMO activity in ethene-grown cultures was not detected after 6.7 h of carbon starvation, while 40% of the initial EaCoMT activity remained after 24 h. Acetate eliminated the extended lag period in starved cultures but not in mid-exponential phase cultures suggesting that acetate reactivates extant AkMO in starved VC- or ethene-grown cultures. The imbalance between AkMO and EaCoMT activities during starvation likely contributes to the extended lag period by delaying epoxide accumulation and subsequent AkMO induction.
المشرفين على المادة: 0 (Ethylenes)
WD06X94M2D (Vinyl Chloride)
تواريخ الأحداث: Date Created: 20070220 Date Completed: 20071101 Latest Revision: 20131121
رمز التحديث: 20231215
DOI: 10.1007/s00203-006-0189-2
PMID: 17308936
قاعدة البيانات: MEDLINE
الوصف
تدمد:0302-8933
DOI:10.1007/s00203-006-0189-2