دورية أكاديمية
أعرض في EDS

Longitudinal analysis of gene expression in porcine skeletal muscle after post-injection local injury.

التفاصيل البيبلوغرافية
العنوان: Longitudinal analysis of gene expression in porcine skeletal muscle after post-injection local injury.
المؤلفون: Ferré PJ; UMR181 de Physiopathologie et Toxicologie Expérimentales, INRA, ENVT, National Veterinary School, 23 chemin des Capelles, BP 87614, Toulouse cedex 03, 31076, France., Liaubet L, Concordet D, SanCristobal M, Uro-Coste E, Tosser-Klopp G, Bonnet A, Toutain PL, Hatey F, Lefebvre HP
المصدر: Pharmaceutical research [Pharm Res] 2007 Aug; Vol. 24 (8), pp. 1480-9. Date of Electronic Publication: 2007 Mar 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 8406521 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0724-8741 (Print) Linking ISSN: 07248741 NLM ISO Abbreviation: Pharm Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : New York, NY : Kluwer Academic/Plenum Publishers
Original Publication: Stuttgart ; New York : Thieme, c1984-
مواضيع طبية MeSH: Gene Expression Profiling*, Muscle Proteins/*genetics , Muscle, Skeletal/*metabolism, Animals ; Collagen/genetics ; Collagen Type I/genetics ; Collagen Type I, alpha 1 Chain ; Down-Regulation/genetics ; Fibronectins/genetics ; Injections, Intramuscular/adverse effects ; Male ; Matrix Metalloproteinase 2/genetics ; Muscle, Skeletal/injuries ; Oligonucleotide Array Sequence Analysis/methods ; Osteonectin/genetics ; Swine ; Thymosin/genetics ; Time Factors ; Up-Regulation/genetics ; ras Proteins/genetics
مستخلص: Purpose: The purpose of this study is to describe the time course of gene expression in a skeletal muscle local injury induced by an intramuscular (IM) injection, and to compare the dynamics of gene expression with pathological events.
Materials and Methods: Ten piglets received 4 IM injections of propylene glycol in the longissimus dorsi muscles 6 h, 2, 7, and 21 days before euthanasia, where control and injected muscle sites were sampled for RNA isolation and microscopic examination. The hybridization of nylon cDNA microarrays was carried out with radioactive probes obtained from the muscle RNA.
Results: 153 genes were found under- or over-expressed at least once among the investigated time-conditions. The eight most discriminant genes were also identified: Two genes (GTP-binding protein RAD and Ankyrin repeat domain protein) were over-expressed at 6 h and six genes between 2 and 21 days (Osteonectin, Fibronectin, Matrix metalloproteinase-2, Collagen alpha 1(I) chain, Collagen alpha 2(I) chain, and Thymosin beta-4). Necrosis, inflammation and regeneration were observed through both the dynamics of gene expression profiles and through the microscopic examinations.
Conclusion: Our data demonstrate that several pathways are involved in post-injection muscle injury, and that necrosis, inflammation and regeneration are not sequential but occur in parallel.
References: J Pediatr. 1967 Jan;70(1):158-9. (PMID: 6016800)
Genome Biol. 2002 Jul 15;3(8):SOFTWARE0003. (PMID: 12186655)
J Mol Biol. 2005 Jun 3;349(2):349-66. (PMID: 15890200)
Mamm Genome. 2002 Aug;13(8):475-8. (PMID: 12226715)
J Invest Dermatol. 1999 Sep;113(3):364-8. (PMID: 10469335)
Nature. 2005 Jun 30;435(7046):1262-6. (PMID: 15988529)
Pharm Res. 1989 Sep;6(9):766-71. (PMID: 2813273)
Nat Genet. 2001 Dec;29(4):365-71. (PMID: 11726920)
Vet Res. 1996;27(4-5):343-61. (PMID: 8822606)
Nat Med. 1999 Dec;5(12):1424-7. (PMID: 10581087)
J Biochem. 2003 Jun;133(6):745-56. (PMID: 12869531)
Brain. 2001 Sep;124(Pt 9):1821-31. (PMID: 11522584)
Am J Physiol Gastrointest Liver Physiol. 2004 Oct;287(4):G875-85. (PMID: 15178550)
BMC Bioinformatics. 2006 Jan 06;7:3. (PMID: 16398926)
BMC Genomics. 2003 Mar 1;4(1):8. (PMID: 12611633)
JAMA. 1978 Aug 11;240(6):542-4. (PMID: 671665)
J Pharm Sci. 1990 May;79(5):393-7. (PMID: 2352156)
Am J Physiol Cell Physiol. 2001 Apr;280(4):C763-8. (PMID: 11245591)
Pediatrics. 1982 Dec;70(6):944-8. (PMID: 6755373)
J Mol Biol. 2004 May 28;339(2):313-25. (PMID: 15136035)
J Interferon Cytokine Res. 2003 May;23(5):237-45. (PMID: 12804066)
Med Sci Sports Exerc. 1991 Jul;23(7):801-10. (PMID: 1921672)
J Bone Joint Surg Br. 1985 Jan;67(1):94-6. (PMID: 3968153)
Am J Vet Res. 2001 Nov;62(11):1698-705. (PMID: 11703010)
BMC Genomics. 2004 Jun 29;5(1):38. (PMID: 15222896)
Matrix Biol. 2000 Dec;19(7):569-80. (PMID: 11102747)
J Bone Joint Surg Am. 1980 Jan;62(1):58-60. (PMID: 7351417)
Am J Physiol Regul Integr Comp Physiol. 2005 Feb;288(2):R345-53. (PMID: 15637171)
PDA J Pharm Sci Technol. 2000 Nov-Dec;54(6):456-69. (PMID: 11107838)
Biochem Biophys Res Commun. 2001 Jul 13;285(2):378-86. (PMID: 11444853)
Schweiz Med Wochenschr. 1995 Aug 8;125(31-32):1477-82. (PMID: 7676237)
Biochem J. 2001 Jun 15;356(Pt 3):737-45. (PMID: 11389681)
J Clin Invest. 2001 May;107(9):1049-54. (PMID: 11342565)
Pediatr Nurs. 1989 Jul-Aug;15(4):333-6, 376. (PMID: 2685732)
Pharm Res. 2005 Mar;22(3):405-12. (PMID: 15835746)
المشرفين على المادة: 0 (Collagen Type I)
0 (Collagen Type I, alpha 1 Chain)
0 (Fibronectins)
0 (Muscle Proteins)
0 (Osteonectin)
549LM7U24W (thymosin beta(4))
61512-21-8 (Thymosin)
9007-34-5 (Collagen)
EC 3.4.24.24 (Matrix Metalloproteinase 2)
EC 3.6.5.2 (ras Proteins)
تواريخ الأحداث: Date Created: 20070324 Date Completed: 20070904 Latest Revision: 20211203
رمز التحديث: 20221213
DOI: 10.1007/s11095-007-9266-8
PMID: 17380264
قاعدة البيانات: MEDLINE
الوصف
تدمد:0724-8741
DOI:10.1007/s11095-007-9266-8