دورية أكاديمية
[Regulation of tyrosylprotein sulfotransferases activity by sulfotyrosine].
| العنوان: | [Regulation of tyrosylprotein sulfotransferases activity by sulfotyrosine]. |
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| المؤلفون: | Gao JM; Department of Respiratory Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. gaojm@pumch.ac.cn, Feng QP, Zuo J, Fang FD, Jiang L, Guo ZJ |
| المصدر: | Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [Zhongguo Yi Xue Ke Xue Yuan Xue Bao] 2007 Apr; Vol. 29 (2), pp. 241-5. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yi xue ke xue yuan Country of Publication: China NLM ID: 8006230 Publication Model: Print Cited Medium: Print ISSN: 1000-503X (Print) Linking ISSN: 1000503X NLM ISO Abbreviation: Zhongguo Yi Xue Ke Xue Yuan Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yi xue ke xue yuan Original Publication: [Beijing] Zhongguo yi xue ke xue yuan. |
| مواضيع طبية MeSH: | Sulfotransferases/*metabolism , Tyrosine/*analogs & derivatives, Cell Line ; Complement C3a/metabolism ; Complement C5a/metabolism ; Humans ; Protein Binding ; Protein Processing, Post-Translational ; Receptor, Anaphylatoxin C5a/metabolism ; Receptors, Complement/metabolism ; Sulfotransferases/genetics ; Transfection ; Tyrosine/metabolism |
| مستخلص: | Objective: To investigate the role of sulfated tyrosine in regulating the activity of tyrosylprotein sulfotransferases (TPST) 1 and TPST2. Methods: Constructs of TPST 1 and TPST2 were amplified by polymerase chain reaction (PCR), then fused into immunoglobulin G1 Fc region. All the variants in which sulfated tyrosines were mutated to phenylalanine were made by the PCR-based Quick Change method and confirmed by sequencing the entire reading frame. Small hairpin RNA (shRNA) constructs-targeting nucleotides 259-275 of TPST1 and nucleotides 73-94 of TPST2 were generated and subcloned into pBluescript. Human embryonic kidney (HEK) 293T cells were transfected with these plasmids. One day later, cells were split: one part was labeled with 35S-cysteine and methionine or 35S-Na2SO3 overnight, the second part was used for 125I labeled binding experiment, and the third part was retained for binding and flow cytometry. Results: Tyrosines at position 326 of TPST1 and position 325 of TPST2 were sulfated posttranslationally. Tyrosine sulfation of TPSTs was effectively inhibited by sulfation inhibitors, including specific shRNAs and non-specific NaCIO3. shRNAs reduced the sulfation of C3a receptor and C5a receptor, and partially blocked the binding of these two receptors to their respective ligands. Conclusions: The activities of TPSTs were regulated by tyrosine sulfation. Inhibition of sulfotyrosine decreases the binding ability of C3a receptor and C5a receptor to their respective ligands. |
| المشرفين على المادة: | 0 (Receptor, Anaphylatoxin C5a) 0 (Receptors, Complement) 0 (complement C3a receptor) 29166358BF (tyrosine O-sulfate) 42HK56048U (Tyrosine) 80295-42-7 (Complement C3a) 80295-54-1 (Complement C5a) EC 2.8.2.- (Sulfotransferases) EC 2.8.2.20 (protein-tyrosine sulfotransferase) |
| تواريخ الأحداث: | Date Created: 20070601 Date Completed: 20100511 Latest Revision: 20151119 |
| رمز التحديث: | 20240628 |
| PMID: | 17536277 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-503X |
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