دورية أكاديمية
Microbial contamination of embryo cultures in an ART laboratory: sources and management.
| العنوان: | Microbial contamination of embryo cultures in an ART laboratory: sources and management. |
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| المؤلفون: | Kastrop PM; Department of Reproductive Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. p.kastrop@umcutrecht.nl, de Graaf-Miltenburg LA, Gutknecht DR, Weima SM |
| المصدر: | Human reproduction (Oxford, England) [Hum Reprod] 2007 Aug; Vol. 22 (8), pp. 2243-8. Date of Electronic Publication: 2007 Jun 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8701199 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0268-1161 (Print) Linking ISSN: 02681161 NLM ISO Abbreviation: Hum Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the European Society of Human Reproduction and Embryology by IRL Press, [c1986- |
| مواضيع طبية MeSH: | Fertilization in Vitro* , Sperm Injections, Intracytoplasmic*, Oocytes/*microbiology , Semen/*microbiology, Candida/isolation & purification ; Embryo Culture Techniques/standards ; Escherichia coli/isolation & purification ; Female ; Humans ; Male ; Retrospective Studies |
| مستخلص: | Background: Although rare, microbial contamination of culture dishes occasionally occurs in our IVF/ICSI programme. Despite stringent culture conditions and the use of medium containing penicillin and streptomycin, an increasing number of infections was observed once they were routinely recorded. In this study, 95 cases of contaminated culture dishes were examined, in an attempt to identify possible causes. Methods: Relevant data of the IVF/ICSI treatment cycles and the micro-organisms isolated from the infected culture dishes were evaluated retrospectively. Results: Infections were observed only in IVF culture dishes and never after applying intra-cytoplasmic sperm injection. Identification of the contaminating micro-organisms showed that infections were mainly caused by Escherichia coli (n = 56; 58.9%) and Candida species (n = 24; 25.3%). Of the E. coli strains isolated, 41 (73.2%) appeared to be resistant to both antibiotics used in the culture medium and 13 (23.2%) appeared to resist either penicillin or streptomycin. Of all bacterial strains isolated, the resistances were 61.4% to both and 30% to one of the antibiotics used. Conclusions: Applying the ICSI procedure prevents colonization of the culture dishes by micro-organisms. Infections in IVF culture dishes are mainly caused by bacterial strains insensitive to the antibiotics used or due to yeast colonization by Candida species which frequently reside in the vagina. |
| تواريخ الأحداث: | Date Created: 20070623 Date Completed: 20071012 Latest Revision: 20070727 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/humrep/dem165 |
| PMID: | 17584750 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0268-1161 |
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| DOI: | 10.1093/humrep/dem165 |