دورية أكاديمية
Immunohistochemistry for PrPSc in natural scrapie reveals patterns which are associated with the PrP genotype.
| العنوان: | Immunohistochemistry for PrPSc in natural scrapie reveals patterns which are associated with the PrP genotype. |
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| المؤلفون: | Spiropoulos J; Neuropathology Unit, Veterinary Laboratories Agency, Weybridge, Surrey, UK. j.spiropoulos@vla.defra.gsi.gov.uk, Casalone C, Caramelli M, Simmons MM |
| المصدر: | Neuropathology and applied neurobiology [Neuropathol Appl Neurobiol] 2007 Aug; Vol. 33 (4), pp. 398-409. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 7609829 Publication Model: Print Cited Medium: Print ISSN: 0305-1846 (Print) Linking ISSN: 03051846 NLM ISO Abbreviation: Neuropathol Appl Neurobiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, Blackwell Scientific Publications. |
| مواضيع طبية MeSH: | PrPSc Proteins/*genetics , PrPSc Proteins/*metabolism , Scrapie/*genetics , Scrapie/*metabolism , Sheep Diseases/*genetics , Sheep Diseases/*metabolism, Animals ; Antibodies, Monoclonal ; Genotype ; Immunohistochemistry ; Neuropil/pathology ; Phenotype ; PrPSc Proteins/chemistry ; Predictive Value of Tests ; Prognosis ; Sheep |
| مستخلص: | Immunohistochemistry for PrPSc is used widely in scrapie diagnosis. In natural scrapie cases the use of immunohistochemistry (IHC) has revealed the existence of up to 12 different morphological types of immunostained deposits. The significance of this pattern variability in relation to genotype has not been studied extensively in natural disease. In this study we recorded in detail PrPSc patterns at the obex level of the medulla oblongata from 163 animals derived from 55 flocks which presented through passive surveillance in the UK and Italy. A strong association was seen between PrPSc patterns and PrP genotype, particularly in relation to codon 136. In a blind assessment of this association we were able to predict, with over 80% accuracy, the genotype of 151 scrapie cases which were presented through passive surveillance from 13 farms. The genotype of these cases was ARQ/ARQ or VRQ/VRQ. The association of PrPsc patterns with genotype was generally stronger in those farms where all the affected animals belonged to a single genotype compared with farms where both genotypes were identified, with the exception of one farm in which the genotype of all affected sheep was ARQ/ARQ and the PrPSc patterns were of the VRQ/VRQ type. Our observations support the hypothesis that the observed association between specific IHC patterns and genotypes may in fact be strain driven but in natural disease individual scrapie strains may demonstrate a genotypic tropism. |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (PrPSc Proteins) |
| تواريخ الأحداث: | Date Created: 20070710 Date Completed: 20070911 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/j.1365-2990.2007.00800.x |
| PMID: | 17617872 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0305-1846 |
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| DOI: | 10.1111/j.1365-2990.2007.00800.x |