دورية أكاديمية
A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method.
| العنوان: | A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method. |
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| المؤلفون: | Camerini S; San Raffaele Scientific Institute, 20132 Milan, Italy. serena.camerini@iss.it, Polci ML, Restuccia U, Usuelli V, Malgaroli A, Bachi A |
| المصدر: | Journal of proteome research [J Proteome Res] 2007 Aug; Vol. 6 (8), pp. 3224-31. Date of Electronic Publication: 2007 Jul 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1535-3893 (Print) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: | Cerebellum/*metabolism , Nerve Tissue Proteins/*analysis , Nitric Oxide/*metabolism , Nitroso Compounds/*chemistry , S-Nitrosoglutathione/*chemistry, Amino Acid Sequence ; Animals ; Female ; Male ; Molecular Sequence Data ; Nerve Tissue Proteins/chemistry ; Nitric Oxide/chemistry ; Ovalbumin/analysis ; Ovalbumin/chemistry ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods |
| مستخلص: | S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis. |
| المشرفين على المادة: | 0 (Nerve Tissue Proteins) 0 (Nitroso Compounds) 31C4KY9ESH (Nitric Oxide) 57564-91-7 (S-Nitrosoglutathione) 9006-59-1 (Ovalbumin) |
| تواريخ الأحداث: | Date Created: 20070717 Date Completed: 20080926 Latest Revision: 20131121 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/pr0701456 |
| PMID: | 17629318 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1535-3893 |
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| DOI: | 10.1021/pr0701456 |