التفاصيل البيبلوغرافية
| العنوان: |
[Somatic hybrids between Rorippa indica (Linn.) Hiern and Brassica napus L. through protoplast-fusion system]. |
| المؤلفون: |
Jiang SH; State Key Lab of Crop Genetics and Germpalsm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. jsh1982@eyou.com, Guan RZ, Tang SY, Xin RY, Zhang HS, Zhao LQ, Pan QY |
| المصدر: |
Yi chuan = Hereditas [Yi Chuan] 2007 Jun; Vol. 29 (6), pp. 745-50. |
| نوع المنشور: |
English Abstract; Journal Article |
| اللغة: |
Chinese |
| بيانات الدورية: |
Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9436478 Publication Model: Print Cited Medium: Print ISSN: 0253-9772 (Print) Linking ISSN: 02539772 NLM ISO Abbreviation: Yi Chuan Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Beijing : Ke xue chu ban she, |
| مواضيع طبية MeSH: |
Brassica napus/*cytology , Protoplasts/*cytology , Rorippa/*cytology, Brassica napus/physiology ; Cell Fusion ; Chimera/physiology ; Protoplasts/physiology ; Regeneration ; Rorippa/physiology |
| مستخلص: |
High yield of protoplast isolation was achieved from hypocotyls of B. napus L. and leaves of Rorippa indica (Linn.) Hiern. The isolated protoplasts were used to establish an efficient protoplast-fusion system between the two cruciferous species by PEG-DMSO method and culturing with MS liquid medium. Ten somatic fusion hybrids between B. napus and R. indica were obtained. The enzyme combinations for isolating protoplast from B. napus L. and R. indica were 1% cellulase + 0.2% macerozyme + 3 mmol/L MES and 0.25% cellulase + 0.5% macerozyme + 5 mmol/L MES, respectively. Fusion percentage of 10.4% was obtained on the condition of 30% PEG + 0.3 mol/L glucose +50 mmol/L CaCl2.2H2O + 15% DMSO. Seeds plants obtained from protoplast fusion are new germplasm derived from R. indica. |
| تواريخ الأحداث: |
Date Created: 20070726 Date Completed: 20090327 Latest Revision: 20190923 |
| رمز التحديث: |
20221213 |
| DOI: |
10.1360/yc-007-0745 |
| PMID: |
17650493 |
| قاعدة البيانات: |
MEDLINE |
