Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes.

التفاصيل البيبلوغرافية
العنوان:	Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes.
المؤلفون:	Mitchell LA; College of Pharmacy, University of New Mexico, Albuquerque, New Mexico 87131-0001, USA., Gao J, Wal RV, Gigliotti A, Burchiel SW, McDonald JD
المصدر:	Toxicological sciences : an official journal of the Society of Toxicology [Toxicol Sci] 2007 Nov; Vol. 100 (1), pp. 203-14. Date of Electronic Publication: 2007 Jul 28.
نوع المنشور:	Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة:	English
بيانات الدورية:	Publisher: Oxford University Press Country of Publication: United States NLM ID: 9805461 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1096-6080 (Print) Linking ISSN: 10960929 NLM ISO Abbreviation: Toxicol Sci Subsets: MEDLINE
أسماء مطبوعة:	Publication: 1999- : Cary, NC : Oxford University Press Original Publication: Orlando, FL : Academic Press, c1998-
مواضيع طبية MeSH:	Inhalation Exposure, Immune System/drug effects , Lung/drug effects , Lymphocyte Activation/drug effects , Nanotubes, Carbon/toxicity , T-Lymphocytes/drug effects, Aerosols ; Animals ; Bronchoalveolar Lavage Fluid/cytology ; Cells, Cultured ; Dose-Response Relationship, Drug ; Gene Expression Regulation/drug effects ; Immune System/metabolism ; Immune System/pathology ; Interleukin-10/metabolism ; Interleukin-6/metabolism ; Killer Cells, Natural/drug effects ; Leukocyte Count ; Lung/metabolism ; Lung/pathology ; Macrophages, Alveolar/drug effects ; Macrophages, Alveolar/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; NAD(P)H Dehydrogenase (Quinone) ; NADPH Dehydrogenase/metabolism ; Oxidative Stress/drug effects ; Particle Size ; RNA, Messenger/metabolism ; Spleen/drug effects ; Spleen/metabolism ; T-Lymphocytes/metabolism ; T-Lymphocytes/pathology ; Time Factors
مستخلص:	Inhalation of multiwalled carbon nanotubes (MWCNTs) at particle concentrations ranging from 0.3 to 5 mg/m3 did not result in significant lung inflammation or tissue damage, but caused systemic immune function alterations. C57BL/6 adult (10- to 12-week) male mice were exposed by whole-body inhalation to control air or 0.3, 1, or 5 mg/m3 respirable aggregates of MWCNTs for 7 or 14 days (6 h/day). Histopathology of lungs from exposed animals showed alveolar macrophages containing black particles; however, there was no inflammation or tissue damage observed. Bronchial alveolar lavage fluid also demonstrated particle-laden macrophages; however, white blood cell counts were not increased compared to controls. MWCNT exposures to 0.3 mg/m3 and higher particle concentrations caused nonmonotonic systemic immunosuppression after 14 days but not after 7 days. Immunosuppression was characterized by reduced T-cell-dependent antibody response to sheep erythrocytes as well as T-cell proliferative ability in presence of mitogen, Concanavalin A. Assessment of nonspecific natural killer (NK) cell activity showed that animals exposed to 1 mg/m(3) had decreased NK cell function. Gene expression analysis of selected cytokines and an indicator of oxidative stress were assessed in lung tissue and spleen. No changes in gene expression were observed in lung; however, interleukin-10 (IL-10) and NAD(P)H oxidoreductase 1 mRNA levels were increased in spleen.
التعليقات:	Comment in: Toxicol Sci. 2008 Jan;101(1):179-80; author reply 181-2. (PMID: 17897971)
معلومات مُعتمدة:	5 S11 ES013339-03 United States ES NIEHS NIH HHS; P30 ES012072 United States ES NIEHS NIH HHS
المشرفين على المادة:	0 (Aerosols) 0 (Interleukin-6) 0 (Nanotubes, Carbon) 0 (RNA, Messenger) 130068-27-8 (Interleukin-10) EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone)) EC 1.6.5.2 (Nqo1 protein, mouse) EC 1.6.99.1 (NADPH Dehydrogenase)
تواريخ الأحداث:	Date Created: 20070731 Date Completed: 20071206 Latest Revision: 20101118
رمز التحديث:	20231215
DOI:	10.1093/toxsci/kfm196
PMID:	17660506
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1096-6080
DOI:	10.1093/toxsci/kfm196