التفاصيل البيبلوغرافية
| العنوان: |
[The preparation of oligonucleotide chips for detecting BMPR-IB gene polymorphism in sheep]. |
| المؤلفون: |
Yang H; College of Animal Science & Technology, Shihezi University, Shihezi 832003, China. yhxjcn@hotmail.com, Zhong FG, Wang XH, Liu SR, Zhu B, Xing JF, Sun Y |
| المصدر: |
Yi chuan = Hereditas [Yi Chuan] 2007 Aug; Vol. 29 (8), pp. 957-62. |
| نوع المنشور: |
English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
Chinese |
| بيانات الدورية: |
Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9436478 Publication Model: Print Cited Medium: Print ISSN: 0253-9772 (Print) Linking ISSN: 02539772 NLM ISO Abbreviation: Yi Chuan Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Beijing : Ke xue chu ban she, |
| مواضيع طبية MeSH: |
Bone Morphogenetic Protein Receptors, Type I/*genetics , Oligonucleotide Array Sequence Analysis/*methods , Polymorphism, Genetic/*genetics , Sheep/*genetics, Animals ; Mutation ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length |
| مستخلص: |
BMPR-IB gene increases ovulation rate and litter size as a major gene in Chinese Merino, because of 746 A to G mutation. The aim of this study was to make oligonucleotide chips to determine single nucleotide polymorphism (SNP) in BMPR-IB gene. The oligonucleotide chips were manufactured by using six sequence-specific oligonucleotide probes derived from polymorphic regions in the mutation and spotting the probes by microarrayer onto the aldehyde modified glass slides. The 746 A to G mutation was detected with ovine blood in reaction cells of chips. The results were identified by designed the Arraydoctor 2.0 software, in accordance with those of restriction fragment length polymorphisms (RFLP). The results showed the oligonucleotide chips are a parallel, accurate and effective way for screening prolificacy in molecular-assisted selection(MAS)of sheep. |
| المشرفين على المادة: |
EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type I) |
| تواريخ الأحداث: |
Date Created: 20070808 Date Completed: 20090327 Latest Revision: 20190923 |
| رمز التحديث: |
20221213 |
| DOI: |
10.1360/yc-007-0957 |
| PMID: |
17681924 |
| قاعدة البيانات: |
MEDLINE |
