دورية أكاديمية
Improved tumor targeting and decreased normal tissue accumulation through extracorporeal affinity adsorption in a two-step pretargeting strategy.
| العنوان: | Improved tumor targeting and decreased normal tissue accumulation through extracorporeal affinity adsorption in a two-step pretargeting strategy. |
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| المؤلفون: | Mårtensson L; Department of Oncology, University of Lund, Sweden. linda.martensson@med.lu.se, Nilsson R, Ohlsson T, Sjögren HO, Strand SE, Tennvall J |
| المصدر: | Clinical cancer research : an official journal of the American Association for Cancer Research [Clin Cancer Res] 2007 Sep 15; Vol. 13 (18 Pt 2), pp. 5572s-5576s. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: The Association Country of Publication: United States NLM ID: 9502500 Publication Model: Print Cited Medium: Print ISSN: 1078-0432 (Print) Linking ISSN: 10780432 NLM ISO Abbreviation: Clin Cancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Denville, NJ : The Association, c1995- |
| مواضيع طبية MeSH: | Extracorporeal Circulation*, Biotin/*analogs & derivatives , Colonic Neoplasms/*diagnostic imaging , Indium Radioisotopes/*pharmacokinetics , Organometallic Compounds/*pharmacokinetics , Streptavidin/*pharmacokinetics, Adsorption ; Animals ; Biotin/metabolism ; Biotin/pharmacokinetics ; Colonic Neoplasms/metabolism ; Heterocyclic Compounds, 1-Ring/chemistry ; Heterocyclic Compounds, 1-Ring/metabolism ; Liver/diagnostic imaging ; Liver/metabolism ; Lymph Nodes/diagnostic imaging ; Lymph Nodes/metabolism ; Pancreas/diagnostic imaging ; Pancreas/metabolism ; Radionuclide Imaging ; Rats ; Rats, Inbred BN ; Tissue Distribution |
| مستخلص: | Purpose: Evaluation of the possibilities of reducing the accumulation of radiolabeled streptavidin in radiosensitive organs by extracorporeal affinity adsorption (ECAT). Experimental Design: Rats were injected with biotinylated antibody and subjected to removal of the antibodies from the circulation by ECAT 24 h after injection (avidin column). Animals were then injected with 111In-1,4,7,10-tetra-azacylododecane N,N',N'',N'''-tetraacetic acid (DOTA)-streptavidin. In a third step, animals were subjected to a second ECAT 8 h after injection to remove the DOTA-streptavidin from the circulation (biotin column). Biodistribution and tumor targeting of DOTA-streptavidin 24 h after injection was determined. Results: Elimination of biotinylated antibody by ECAT before injection of DOTA-streptavidin increased the tumor targeting by 50%. In addition, the levels of DOTA-streptavidin in liver and lymph nodes were reduced by 60%, which implied a 4.3- and 3.8-fold increase of tumor-to-liver and tumor-to-lymph node ratios, respectively. By doing a second ECAT to remove DOTA-streptavidin from the circulation, accumulation in normal tissues was reduced. However, this latter ECAT also reduced tumor accumulation by 25% (mostly corresponding to radioactivity in the circulation). Conclusions: ECAT was efficient as a means of removing biotinylated antibodies and would probably also be efficient for the clearance of streptavidin-conjugated antibodies. Conversely, the use of ECAT for removal of radiolabeled streptavidin seems not to offer any advantage. |
| المشرفين على المادة: | 0 (DOTA-biotin) 0 (Heterocyclic Compounds, 1-Ring) 0 (Indium Radioisotopes) 0 (Organometallic Compounds) 1HTE449DGZ (1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid) 6SO6U10H04 (Biotin) 9013-20-1 (Streptavidin) |
| تواريخ الأحداث: | Date Created: 20070919 Date Completed: 20071214 Latest Revision: 20161124 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1158/1078-0432.CCR-07-0891 |
| PMID: | 17875791 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1078-0432 |
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| DOI: | 10.1158/1078-0432.CCR-07-0891 |