دورية أكاديمية
Comparison of two automated nucleic acid testing systems for simultaneous detection of human immunodeficiency virus and hepatitis C virus RNA and hepatitis B virus DNA.
| العنوان: | Comparison of two automated nucleic acid testing systems for simultaneous detection of human immunodeficiency virus and hepatitis C virus RNA and hepatitis B virus DNA. |
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| المؤلفون: | Margaritis AR; Australian Red Cross Blood Service-Enterprise, 97 Great Eastern Highway, Rivervale, Western Australia 6103. amargaritis@arcbs.redcross.org.au, Brown SM, Seed CR, Kiely P, D'Agostino B, Keller AJ |
| المصدر: | Transfusion [Transfusion] 2007 Oct; Vol. 47 (10), pp. 1783-93. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association Of Blood Banks Country of Publication: United States NLM ID: 0417360 Publication Model: Print Cited Medium: Print ISSN: 0041-1132 (Print) Linking ISSN: 00411132 NLM ISO Abbreviation: Transfusion Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Arlington, Va. : American Association Of Blood Banks |
| مواضيع طبية MeSH: | Blood Transfusion/*standards , DNA, Viral/*isolation & purification , HIV/*isolation & purification , Hepacivirus/*isolation & purification , Hepatitis B virus/*isolation & purification , RNA, Viral/*blood, Australia ; Automation ; DNA, Viral/genetics ; HIV/genetics ; Hepacivirus/genetics ; Hepatitis B virus/genetics ; Hong Kong ; Humans |
| مستخلص: | Background: Recently developed nucleic acid testing (NAT) assays incorporating simultaneous detection of human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) have made HBV NAT screening more feasible for blood services. This study compared the performance of two "multiplex" NAT assays and their automated testing platforms. Study Design and Methods: The HBV NAT yield rate was estimated by testing 10,397 Hong Kong (HK) donor samples concurrently on the PROCLEIX ULTRIO (Ultrio) assay as individual donor samples with the TIGRIS and on the cobas TaqScreen multiplex (cobas MPX) test in pools of 6 with the cobas s 201. Analytical sensitivity was assessed by probit analysis of diluted international standards and operational performance was compared. Results: Each system detected two different HBV NAT yield samples for a combined rate of 0.04 percent. One additional sample was reactive on the cobas MPX test but remained unresolved. The 95 percent detection limits for HIV-1, HBV, and HCV were 42.2, 12.2, and 2.0 IU per mL, respectively, for Ultrio and 50.5, 8.4, and 6.0 IU per mL for the cobas MPX. The invalid test and failed run rates were 0.05 and 2.92 percent, respectively, for the TIGRIS and 2.39 and 5.53 percent for the cobas s 201. Conclusion: Clinical sensitivity for HBV in HK blood donors was equivalent, as was the analytical sensitivity for HIV-1 and HBV; however, the Ultrio assay had a higher analytical sensitivity for HCV. Despite a shorter downtime and mean time of repair for the cobas s 201, the TIGRIS demonstrated better overall operational performance. |
| المشرفين على المادة: | 0 (DNA, Viral) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20070921 Date Completed: 20071113 Latest Revision: 20070920 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/j.1537-2995.2007.01343.x |
| PMID: | 17880602 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0041-1132 |
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| DOI: | 10.1111/j.1537-2995.2007.01343.x |