دورية أكاديمية
[Replicative kinetics of classical swine fever virus in PK-15 cells].
| العنوان: | [Replicative kinetics of classical swine fever virus in PK-15 cells]. |
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| المؤلفون: | Xu XR; Institute of Military Veterinary, Academy of Military Medical Science Changchun 130062, China., Guo HC, Shi ZX, Xia XZ, Tu CC |
| المصدر: | Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2007 Oct; Vol. 47 (5), pp. 800-4. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Science Press Country of Publication: China NLM ID: 21610860R Publication Model: Print Cited Medium: Print ISSN: 0001-6209 (Print) Linking ISSN: 00016209 NLM ISO Abbreviation: Wei Sheng Wu Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Science Press Original Publication: [Beijing, 1953?]- |
| مواضيع طبية MeSH: | Virus Replication*, Classical Swine Fever Virus/*physiology, Animals ; Cells, Cultured ; Classical Swine Fever Virus/pathogenicity ; Fluorescent Antibody Technique, Indirect ; Kinetics ; RNA, Viral/analysis ; RNA, Viral/biosynthesis ; Swine ; Time Factors |
| مستخلص: | In order to understand the replication kinetics of classical swine fever virus (CSFV) in in vitro cells PK-15 cells were seeded in 96-well tissues culture plates. After overnight incubation at 37 degrees C in 5% CO2 environment when growing to 80% confluence, the cells were infected with CSFV strain Shimen at 100 TCID50 per well. At various time post infection (p.i.) the replication of the virus in the cells were analyzed repectively by detection of viral antigen using indirect immunofluorescent assay (IFA), RNA replication using reverse transcription real-time PCR and viral production using titration of TCID50. In the results of the IFA the viral antigen could be detected as early as 8hrs p.i. and at 72h hrs p.i. almost all cells showed positive staining, the real-time PCR showed that the synthesis of viral genomic RNA was gradually increased between 8-24 hrs p.i. and reached its peak at 72 hrs p.i.. However, the synthesis of negative strand RNA was maintained at a low level for a whole period of culture although it could be detected at 8hrs p.i.. Titration of TCID50 demonstrated that the production of live virions increased at 8h and peaked between 48 - 72 hrs p.i. without significant lose of titer. |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20071208 Date Completed: 20090512 Latest Revision: 20181201 |
| رمز التحديث: | 20231215 |
| PMID: | 18062252 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0001-6209 |
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