دورية أكاديمية
Non-invasive diagnosis of acute rejection in kidney transplants with delayed graft function.
| العنوان: | Non-invasive diagnosis of acute rejection in kidney transplants with delayed graft function. |
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| المؤلفون: | Aquino-Dias EC; Post-Graduate Medical Sciences-Nephrology Program, School of Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Joelsons G, da Silva DM, Berdichevski RH, Ribeiro AR, Veronese FJ, Gonçalves LF, Manfro RC |
| المصدر: | Kidney international [Kidney Int] 2008 Apr; Vol. 73 (7), pp. 877-84. Date of Electronic Publication: 2008 Jan 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0323470 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1755 (Electronic) Linking ISSN: 00852538 NLM ISO Abbreviation: Kidney Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : New York : Elsevier Original Publication: New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Kidney Transplantation*, Delayed Graft Function/*diagnosis , Graft Rejection/*diagnosis, Acute Disease ; Adult ; Biopsy ; Female ; Humans ; Male ; Middle Aged |
| مستخلص: | Delayed graft function (DGF) often occurs in kidney transplants from deceased donors. We wanted to provide studies giving more accurate non-invasive tests for acute rejection (AR). Using real-time PCR, we examined the expression of cytolytic molecules such as perforin, granzyme B, and fas-ligand along with serpin proteinase inhibitor-9. We also measured the expression of FOXP3, a characteristic gene of T-regulatory cells known to be involved in AR. These studies were conducted on peripheral blood monocytes, urinary cells, and 48 surveillance kidney biopsies taken from a total of 35 patients with DGF. Of these patients, 20 had a histopathological diagnosis of AR, whereas other 28 had characteristics of acute tubular necrosis (ATN). Expression of cytolytic and apoptotic-associated genes in the biopsy tissue, peripheral blood leukocytes, and urinary cells was significantly higher in patients with AR than that in patients with ATN. Diagnostic parameters associated with FOXP3 gene expression were most accurate in peripheral blood leukocytes and urine cells with sensitivity, specificity, positive and negative predictive values, and accuracy between 94 and 100%. Our study shows that quantification of selected genes in peripheral blood leukocytes and urinary cells from renal transplant patients with DGF may provide a useful and accurate non-invasive diagnosis of AR. |
| التعليقات: | Erratum in: Kidney Int. 2008 Aug;74(3):393. Veronose, F J V [corrected to Veronese, F J V]; Berdichewski, R H [corrected to Berdichevski, R H]. |
| تواريخ الأحداث: | Date Created: 20080125 Date Completed: 20080804 Latest Revision: 20080805 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1038/sj.ki.5002795 |
| PMID: | 18216781 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1523-1755 |
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| DOI: | 10.1038/sj.ki.5002795 |