دورية أكاديمية
Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation.
| العنوان: | Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation. |
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| المؤلفون: | Zalzal SF; Laboratory for the Study of Calcified Tissues and Biomaterials, Faculty of Dentistry, Université de Montréal, Montreal, QC, Canada., Smith CE, Nanci A |
| المصدر: | Matrix biology : journal of the International Society for Matrix Biology [Matrix Biol] 2008 May; Vol. 27 (4), pp. 352-9. Date of Electronic Publication: 2008 Jan 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9432592 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0945-053X (Print) Linking ISSN: 0945053X NLM ISO Abbreviation: Matrix Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Stuttgart ; New York : Fischer, c1994- |
| مواضيع طبية MeSH: | Amelogenin/*metabolism , Dental Enamel/*metabolism , Dental Enamel Proteins/*metabolism, Animals ; Male ; Microscopy, Electron, Transmission ; Microscopy, Immunoelectron ; Rats ; Rats, Wistar |
| مستخلص: | The epithelially-derived ameloblasts secrete two main categories of extracellular matrix proteins, amelogenins (AMEL) and nonamelogenins. These proteins assume differential distributions in the forming enamel layer and thereby regulate deposition and structuring of the mineral phase. The objective of this study was to elucidate whether their distribution results from distinctive physicochemical behaviors or differences in intracellular routing. Dual-immunogold labeling was used to visualize the presence of AMEL and ameloblastin (AMBN), the major nonamelogenin, and quantify the proportion of secretory granules containing one or both of these proteins in ameloblasts during the phase of appositional growth of the enamel layer in continuously-erupting rat incisors. Some rats were treated with brefeldin A (BFA) to generate a synchronized cohort of newly-formed secretory granules. The results show that nearly 70% of granules contain both AMEL and AMBN, 13% label only for AMBN and 1% only for AMEL. These proportions reach 98% (AMEL+AMBN) and 2% (AMBN only) following BFA treatment. The observation that AMEL is almost always packaged with AMBN suggests a functional association between these two proteins. The subpopulation of granules containing only AMBN could be responsible for augmenting its local concentration along secretory surfaces against which hydroxyapatite crystals actively elongate. |
| معلومات مُعتمدة: | 64178-1 Canada CAPMC CIHR |
| المشرفين على المادة: | 0 (Ambn protein, rat) 0 (Amelogenin) 0 (Dental Enamel Proteins) |
| تواريخ الأحداث: | Date Created: 20080219 Date Completed: 20081031 Latest Revision: 20220224 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.matbio.2008.01.003 |
| PMID: | 18281204 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0945-053X |
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| DOI: | 10.1016/j.matbio.2008.01.003 |