دورية أكاديمية
Differential expression of signal transduction factors in ovarian follicle development: a functional role for betaglycan and FIBP in granulosa cells in cattle.
| العنوان: | Differential expression of signal transduction factors in ovarian follicle development: a functional role for betaglycan and FIBP in granulosa cells in cattle. |
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| المؤلفون: | Forde N; School of Agriculture Food Science and Veterinary Medicine and Conway Institute for Biomolecular and Biomedical Research, College of Life Sciences, University College Dublin, Belfield, Dublin, Ireland., Mihm M, Canty MJ, Zielak AE, Baker PJ, Park S, Lonergan P, Smith GW, Coussens PM, Ireland JJ, Evans AC |
| المصدر: | Physiological genomics [Physiol Genomics] 2008 Apr 22; Vol. 33 (2), pp. 193-204. Date of Electronic Publication: 2008 Feb 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 9815683 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-2267 (Electronic) Linking ISSN: 10948341 NLM ISO Abbreviation: Physiol Genomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1999- |
| مواضيع طبية MeSH: | Gene Expression Profiling* , Gene Expression Regulation*, Carrier Proteins/*genetics , Granulosa Cells/*metabolism , Proteoglycans/*genetics , Receptors, Transforming Growth Factor beta/*genetics , Signal Transduction/*genetics, Animals ; Calcium-Calmodulin-Dependent Protein Kinase Type 1/genetics ; Calcium-Calmodulin-Dependent Protein Kinase Type 1/metabolism ; Carrier Proteins/metabolism ; Cattle ; Cells, Cultured ; Ephrins/genetics ; Ephrins/metabolism ; Estradiol/metabolism ; Female ; Granulosa Cells/cytology ; Progesterone/metabolism ; Proteoglycans/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering ; Receptors, FSH/genetics ; Receptors, FSH/metabolism ; Receptors, LH/genetics ; Receptors, LH/metabolism ; Receptors, Transforming Growth Factor beta/metabolism ; Theca Cells/enzymology |
| مستخلص: | Ovarian follicles develop in groups yet individual follicles follow different growth trajectories. This growth and development are regulated by endocrine and locally produced growth factors that use a myriad of receptors and signal transduction pathways to exert their effects on theca and granulosa cells. We hypothesize that differential growth may be due to differences in hormonal responsiveness that is partially mediated by differences in expression of genes involved in signal transduction. We used the bovine dominant follicle model, microarrays, quantitative real-time PCR and RNA interference to examine this. We identified 83 genes coding for signal transduction molecules and validated a subset of them associated with different stages of the follicle wave. We suggest important roles for CAM kinase-1 and EphA4 in theca cells and BCAR1 in granulosa cells for the development of dominant follicles and for betaglycan and FIBP in granulosa cells of regressing subordinate follicles. Inhibition of genes for betaglycan and FIBP in granulosa cells in vitro suggests that they inhibit estradiol production in regressing subordinate follicles. |
| المشرفين على المادة: | 0 (Carrier Proteins) 0 (Ephrins) 0 (Proteoglycans) 0 (RNA, Messenger) 0 (RNA, Small Interfering) 0 (Receptors, FSH) 0 (Receptors, LH) 0 (Receptors, Transforming Growth Factor beta) 145170-29-2 (betaglycan) 4G7DS2Q64Y (Progesterone) 4TI98Z838E (Estradiol) EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 1) |
| تواريخ الأحداث: | Date Created: 20080221 Date Completed: 20080522 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1152/physiolgenomics.00274.2007 |
| PMID: | 18285519 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1531-2267 |
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| DOI: | 10.1152/physiolgenomics.00274.2007 |