دورية أكاديمية
Neuronal nitric oxide synthase does not contribute to the modulation of pulmonary vascular tone in fetal lambs with congenital diaphragmatic hernia (nNOS in CDH lambs).
| العنوان: | Neuronal nitric oxide synthase does not contribute to the modulation of pulmonary vascular tone in fetal lambs with congenital diaphragmatic hernia (nNOS in CDH lambs). |
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| المؤلفون: | de Buys Roessingh AS; Faculté de Médecine, Université Paris Descartes, EA 2511, Assistance Publique Hôpitaux de Paris, Service de Physiologie-Explorations Fonctionnelles, Hôpital Cochin, Paris, France. anthony.debuys-roessingh@chuv.ch, de Lagausie P, Ebrahimian T, Duong-Quy S, Schneider JC, Huang XL, Mercier JC, Aigrain Y, Boulanger C, Dinh-Xuan AT |
| المصدر: | Pediatric pulmonology [Pediatr Pulmonol] 2008 Apr; Vol. 43 (4), pp. 313-21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8510590 Publication Model: Print Cited Medium: Internet ISSN: 1099-0496 (Electronic) Linking ISSN: 10990496 NLM ISO Abbreviation: Pediatr Pulmonol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005-> : Hoboken, NJ : Wiley-Liss Original Publication: [Philadelphia, PA] : W.B. Saunders, [c1985- |
| مواضيع طبية MeSH: | Hernia, Diaphragmatic/*physiopathology , Lung/*physiopathology , Nitric Oxide Synthase Type I/*metabolism , Pulmonary Artery/*physiopathology, Animals ; Blood Pressure/drug effects ; Blotting, Western ; Disease Models, Animal ; Enzyme Inhibitors/pharmacology ; Fetus/drug effects ; Fetus/enzymology ; Gestational Age ; Heart Rate/drug effects ; Hernia, Diaphragmatic/enzymology ; Hernias, Diaphragmatic, Congenital ; Lung/blood supply ; Lung/drug effects ; Nitric Oxide Synthase Type I/antagonists & inhibitors ; Organ Culture Techniques ; Pulmonary Artery/drug effects ; Pulmonary Artery/enzymology ; Pulmonary Circulation/drug effects ; Sheep ; Vascular Resistance/drug effects |
| مستخلص: | Aim: The aim of this study was to determine the presence of the neuronal nitric oxide synthase (nNOS) in near full-term lambs with congenital diaphragmatic hernia (CDH) and its role in the modulation of pulmonary vascular basal tone. Methods: We surgically created diaphragmatic hernia on the 85th day of gestation. On the 135th, catheters were used to measure pulmonary pressure and blood flow. We tested the effects of 7-nitroindazole (7-NINA), a specific nNOS antagonist and of N-nitro-L-arginine (L-NNA), a nonspecific nitric oxide synthase antagonist. In vitro, we tested the effects of the same drugs on isolated pulmonary vessels. The presence of nNOS protein in the lungs was detected by Western blot analysis. Results: Neither 7-NINA nor L-NNA modified pulmonary vascular basal tone in vivo. After L-NNA injection, acetylcholine (ACh) did not decrease significantly pulmonary vascular resistance (PVR). In vitro, L-NNA increased the cholinergic contractile-response elicited by electric field stimulation (EFS) of vascular rings from lambs with diaphragmatic hernia. Conclusion: We conclude that nNOS protein is present in the lungs and pulmonary artery of near full-term lamb fetuses with diaphragmatic hernia, but that it does not contribute to the reduction of pulmonary vascular tone at birth. |
| التعليقات: | Erratum in: Pediatr Pulmonol. 2008 Jul;43(7):727-8. Ibrahima, Taline [corrected to Ebrahimian, Taline]. |
| المشرفين على المادة: | 0 (Enzyme Inhibitors) EC 1.14.13.39 (Nitric Oxide Synthase Type I) |
| تواريخ الأحداث: | Date Created: 20080229 Date Completed: 20080604 Latest Revision: 20141120 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/ppul.20796 |
| PMID: | 18306337 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-0496 |
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| DOI: | 10.1002/ppul.20796 |