دورية أكاديمية
Interactions between food-borne pathogens and protozoa isolated from lettuce and spinach.
| العنوان: | Interactions between food-borne pathogens and protozoa isolated from lettuce and spinach. |
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| المؤلفون: | Gourabathini P; Center for the Management, Utilization, and Protection of Water Resources, Tennessee Technological University, Cookeville, TN 38505, USA. sberk@tntech.edu, Brandl MT, Redding KS, Gunderson JH, Berk SG |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2008 Apr; Vol. 74 (8), pp. 2518-25. Date of Electronic Publication: 2008 Feb 29. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Escherichia coli O157/*physiology , Eukaryota/*microbiology , Lactuca/*microbiology , Lactuca/*parasitology , Listeria monocytogenes/*physiology , Salmonella enterica/*physiology , Spinacia oleracea/*microbiology , Spinacia oleracea/*parasitology, Animals ; Cell Count ; Colony Count, Microbial ; Escherichia coli O157/growth & development ; Escherichia coli O157/isolation & purification ; Eukaryota/growth & development ; Eukaryota/isolation & purification ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Listeria monocytogenes/growth & development ; Listeria monocytogenes/isolation & purification ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Microscopy, Fluorescence ; Salmonella enterica/growth & development ; Salmonella enterica/isolation & purification ; Staining and Labeling ; Transport Vesicles/microbiology ; Red Fluorescent Protein |
| مستخلص: | The survival of Salmonella enterica was recently shown to increase when the bacteria were sequestered in expelled food vacuoles (vesicles) of Tetrahymena. Because fresh produce is increasingly linked to outbreaks of enteric illness, the present investigation aimed to determine the prevalence of protozoa on spinach and lettuce and to examine their interactions with S. enterica, Escherichia coli O157:H7, and Listeria monocytogenes. Glaucoma sp., Colpoda steinii, and Acanthamoeba palestinensis were cultured from store-bought spinach and lettuce and used in our study. A strain of Tetrahymena pyriformis previously isolated from spinach and a soil-borne Tetrahymena sp. were also used. Washed protozoa were allowed to graze on green fluorescent protein- or red fluorescent protein-labeled enteric pathogens. Significant differences in interactions among the various protist-enteric pathogen combinations were observed. Vesicles were produced by Glaucoma with all of the bacterial strains, although L. monocytogenes resulted in the smallest number per ciliate. Vesicle production was observed also during grazing of Tetrahymena on E. coli O157:H7 and S. enterica but not during grazing on L. monocytogenes, in vitro and on leaves. All vesicles contained intact fluorescing bacteria. In contrast, C. steinii and the amoeba did not produce vesicles from any of the enteric pathogens, nor were pathogens trapped within their cysts. Studies of the fate of E. coli O157:H7 in expelled vesicles revealed that by 4 h after addition of spinach extract, the bacteria multiplied and escaped the vesicles. The presence of protozoa on leafy vegetables and their sequestration of enteric bacteria in vesicles indicate that they may play an important role in the ecology of human pathogens on produce. |
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| المشرفين على المادة: | 0 (Luminescent Proteins) 147336-22-9 (Green Fluorescent Proteins) |
| تواريخ الأحداث: | Date Created: 20080304 Date Completed: 20080513 Latest Revision: 20240109 |
| رمز التحديث: | 20240109 |
| مُعرف محوري في PubMed: | PMC2293137 |
| DOI: | 10.1128/AEM.02709-07 |
| PMID: | 18310421 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
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| DOI: | 10.1128/AEM.02709-07 |