دورية أكاديمية
Inhibition of interleukin-12 production by Trypanosoma brucei in rat macrophages.
| العنوان: | Inhibition of interleukin-12 production by Trypanosoma brucei in rat macrophages. |
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| المؤلفون: | Nishimura K; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. nisimura@vet.osakafu-u.ac.jp, Sakakibara S, Mitani K, Yamate J, Ohnishi Y, Yamasaki S |
| المصدر: | The Journal of parasitology [J Parasitol] 2008 Feb; Vol. 94 (1), pp. 99-106. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Parasitologists [etc.] Country of Publication: United States NLM ID: 7803124 Publication Model: Print Cited Medium: Print ISSN: 0022-3395 (Print) Linking ISSN: 00223395 NLM ISO Abbreviation: J Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lawrence, Kans. [etc.] American Society of Parasitologists [etc.] |
| مواضيع طبية MeSH: | Interleukin-12/*antagonists & inhibitors , Macrophages/*immunology , Trypanosoma brucei gambiense/*immunology, Animals ; Coculture Techniques ; Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis ; Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Immunity, Cellular ; Interferon-gamma/biosynthesis ; Interferon-gamma/genetics ; Interleukin-10/biosynthesis ; Interleukin-10/genetics ; Interleukin-12/biosynthesis ; Interleukin-12/genetics ; Kupffer Cells/cytology ; Macrophage Colony-Stimulating Factor/biosynthesis ; Macrophage Colony-Stimulating Factor/genetics ; Macrophages/parasitology ; Male ; RNA, Messenger/analysis ; Rats ; Rats, Wistar ; Spleen/cytology ; Spleen/immunology ; Tumor Necrosis Factor-alpha/biosynthesis ; Tumor Necrosis Factor-alpha/genetics |
| مستخلص: | The immune response of a host infected with Trypanosoma brucei is modulated by trypomastigotes. We examined the changes in cytokine production in T. brucei gambiense (Wellcome strain; WS) infected rats and the influence on production of interleukin (IL)-12 by macrophages. The blood concentration of interferon-gamma, tumor necrosis factor-alpha, and IL-10 increased beginning the second day after infection. However, an increase in IL-12p40 was not observed until 4 days after infection. When spleen macrophages and Kupffer cells harvested from uninfected rats and HS-P cells (a rat macrophagelike cell line) were cocultured with WS, IL-12p40 production did not change. When HS-P cells were cultured with WS, transport of nuclear factor-kappaB into the nucleus increased. Levels of macrophage colony-stimulating factor (M-CSF) and granulocyte macrophage colony-stimulating factor mRNA in the spleens and livers of WS-infected rats were high in comparison with uninfected rats, suggesting that the WS promotes macrophage proliferation. The level of IL-12p40 mRNA in HS-P cells cocultured with WS increased in response to transfection with a small interfering RNA against M-CSF or addition of anti-M-CSF antibody. These results suggest that the WS inhibits IL-12p40 mRNA production by promoting production of macrophage colony-stimulating factor by macrophages. |
| المشرفين على المادة: | 0 (RNA, Messenger) 0 (Tumor Necrosis Factor-alpha) 130068-27-8 (Interleukin-10) 187348-17-0 (Interleukin-12) 81627-83-0 (Macrophage Colony-Stimulating Factor) 82115-62-6 (Interferon-gamma) 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor) |
| تواريخ الأحداث: | Date Created: 20080401 Date Completed: 20080411 Latest Revision: 20081121 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1645/GE-1322.1 |
| PMID: | 18372627 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0022-3395 |
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| DOI: | 10.1645/GE-1322.1 |