دورية أكاديمية
Effect of Veillonella parvula on the antimicrobial resistance and gene expression of Streptococcus mutans grown in a dual-species biofilm.
| العنوان: | Effect of Veillonella parvula on the antimicrobial resistance and gene expression of Streptococcus mutans grown in a dual-species biofilm. |
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| المؤلفون: | Luppens SB; Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam and Vrije Universiteit, Amsterdam, The Netherlands. s.luppens@acta.nl, Kara D, Bandounas L, Jonker MJ, Wittink FR, Bruning O, Breit TM, Ten Cate JM, Crielaard W |
| المصدر: | Oral microbiology and immunology [Oral Microbiol Immunol] 2008 Jun; Vol. 23 (3), pp. 183-9. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Munksgaard Country of Publication: Denmark NLM ID: 8707451 Publication Model: Print Cited Medium: Internet ISSN: 1399-302X (Electronic) Linking ISSN: 09020055 NLM ISO Abbreviation: Oral Microbiol Immunol |
| أسماء مطبوعة: | Original Publication: Copenhagen : Munksgaard, c1986- |
| مواضيع طبية MeSH: | Biofilms*/growth & development, Anti-Infective Agents/*pharmacology , Drug Resistance, Bacterial/*genetics , Gene Expression Regulation, Bacterial/*genetics , Streptococcus mutans/*physiology , Veillonella/*physiology, Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents, Local/pharmacology ; Cariostatic Agents/pharmacology ; Cetylpyridinium/pharmacology ; Chlorides/pharmacology ; Diamines/pharmacology ; Erythromycin/pharmacology ; Fluorides ; Gene Expression Profiling ; Humans ; Hydrogen Peroxide/pharmacology ; Oligonucleotide Array Sequence Analysis ; Oxidants/pharmacology ; Polysaccharides, Bacterial/genetics ; RNA, Bacterial/genetics ; Streptococcus mutans/drug effects ; Streptococcus mutans/genetics ; Transcription, Genetic/genetics ; Veillonella/drug effects ; Zinc Compounds/pharmacology |
| مستخلص: | Introduction: Our previous studies showed that Streptococcus mutans and Veillonella parvula dual-species biofilms have a different acid production profile and a higher resistance to chlorhexidine than their single-species counterparts. The aim of the current study was to test whether the susceptibility of S. mutans grown in the presence of V. parvula is also decreased when it is exposed to various other antimicrobials. Furthermore, the aim was to identify other changes in the physiology of S. mutans when V. parvula was present using transcriptomics. Methods: Susceptibility to antimicrobials was assessed in killing experiments. Transcript levels in S. mutans were measured with the help of S. mutans microarrays. Results: When V. parvula was present, S. mutans showed an increase in survival after exposure to various antimicrobials. Furthermore, this co-existence altered the physiology of S. mutans. The expression of genes coding for proteins involved in amino acid synthesis, the signal recognition particle-translocation pathway, purine metabolism, intracellular polysaccharide synthesis, and protein synthesis all changed. Conclusion: Growing in a biofilm together with a non-pathogenic bacterium like V. parvula changes the physiology of S. mutans, and gives this bacterium an advantage in surviving antimicrobial treatment. Thus, the study of pathogens implicated in polymicrobial diseases, such as caries and periodontitis, should be focused more on multispecies biofilms. In addition, the testing of susceptibility to currently used and new antimicrobials should be performed on a multispecies microbial community rather than with single pathogens. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Anti-Infective Agents) 0 (Anti-Infective Agents, Local) 0 (Cariostatic Agents) 0 (Chlorides) 0 (Diamines) 0 (Oxidants) 0 (Polysaccharides, Bacterial) 0 (RNA, Bacterial) 0 (Zinc Compounds) 63937KV33D (Erythromycin) 86Q357L16B (zinc chloride) 8NY9L8837D (Olaflur) BBX060AN9V (Hydrogen Peroxide) CUB7JI0JV3 (Cetylpyridinium) Q80VPU408O (Fluorides) |
| تواريخ الأحداث: | Date Created: 20080412 Date Completed: 20080708 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1111/j.1399-302X.2007.00409.x |
| PMID: | 18402603 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1399-302X |
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| DOI: | 10.1111/j.1399-302X.2007.00409.x |