دورية أكاديمية
Pressure-inactivated yellow fever 17DD virus: implications for vaccine development.
| العنوان: | Pressure-inactivated yellow fever 17DD virus: implications for vaccine development. |
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| المؤلفون: | Gaspar LP; Programa de Vacinas Virais, Instituto de Tecnologia em Imunobiológicos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. lgaspar@bio.fiocruz.br, Mendes YS, Yamamura AM, Almeida LF, Caride E, Gonçalves RB, Silva JL, Oliveira AC, Galler R, Freire MS |
| المصدر: | Journal of virological methods [J Virol Methods] 2008 Jun; Vol. 150 (1-2), pp. 57-62. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print Cited Medium: Print ISSN: 0166-0934 (Print) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Virus Inactivation*, Yellow Fever Vaccine/*adverse effects , Yellow Fever Vaccine/*immunology , Yellow fever virus/*physiology, Animals ; Antibodies, Viral/blood ; Chlorocebus aethiops ; Hydrostatic Pressure ; Mice ; Microbial Viability ; Neutralization Tests ; Survival Analysis ; Vero Cells ; Viral Plaque Assay ; Yellow Fever/virology ; Yellow fever virus/immunology |
| مستخلص: | The successful Yellow Fever (YF) vaccine consists of the live attenuated 17D-204 or 17DD viruses. Despite its excellent record of efficacy and safety, serious adverse events have been recorded and influenced extensive vaccination in endemic areas. Therefore, alternative strategies should be considered, which may include inactivated whole virus. High hydrostatic pressure has been described as a method for viral inactivation and vaccine development. The present study evaluated whether high hydrostatic pressure would inactivate the YF 17DD virus. YF 17DD virus was grown in Vero cells in roller bottle cultures and subjected to 310MPa for 3h at 4 degrees C. This treatment abolished YF infectivity and eliminated the ability of the virus to cause disease in mice. Pressure-inactivated virus elicited low level of neutralizing antibody titers although exhibited complete protection against an otherwise lethal challenge with 17DD virus in the murine model. The data warrant further development of pressure-inactivated vaccine against YF. |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Yellow Fever Vaccine) |
| تواريخ الأحداث: | Date Created: 20080419 Date Completed: 20080717 Latest Revision: 20191210 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jviromet.2008.03.002 |
| PMID: | 18420285 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0166-0934 |
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| DOI: | 10.1016/j.jviromet.2008.03.002 |