دورية أكاديمية
ERK1/2 activation is required for resveratrol-induced apoptosis in MDA-MB-231 cells.
| العنوان: | ERK1/2 activation is required for resveratrol-induced apoptosis in MDA-MB-231 cells. |
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| المؤلفون: | Nguyen TH; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456., Mustafa FB, Pervaiz S, Ng FS, Lim LH |
| المصدر: | International journal of oncology [Int J Oncol] 2008 Jul; Vol. 33 (1), pp. 81-92. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: D.A. Spandidos Country of Publication: Greece NLM ID: 9306042 Publication Model: Print Cited Medium: Print ISSN: 1019-6439 (Print) Linking ISSN: 10196439 NLM ISO Abbreviation: Int J Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2003->: Athens, Greece : D.A. Spandidos Original Publication: Athens, Greece : Lychnia, |
| مواضيع طبية MeSH: | Anticarcinogenic Agents/*pharmacology , Apoptosis/*drug effects , Breast Neoplasms/*drug therapy , Mitogen-Activated Protein Kinase 1/*metabolism , Mitogen-Activated Protein Kinase 3/*metabolism , Stilbenes/*pharmacology, Breast Neoplasms/pathology ; Cell Line, Tumor ; Enzyme Activation ; Female ; Humans ; Mitogen-Activated Protein Kinase 1/antagonists & inhibitors ; Mitogen-Activated Protein Kinase 3/antagonists & inhibitors ; Phosphatidylinositol 3-Kinases/physiology ; Poly(ADP-ribose) Polymerases/metabolism ; Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors ; RNA, Small Interfering/pharmacology ; Resveratrol |
| مستخلص: | Resveratrol (RSVL), a phytoalexin found in abundance in grapes and other grape-related products, has been shown to be antiproliferative and protective against various types of cancers, including breast cancer. However, the precise underlying mechanisms are not well understood. In this study, we show that treatment with RSVL induces growth inhibition and apoptosis in a highly invasive and metastatic breast cancer cell line MDA-MB-231. Cleavage of caspase-3 and PARP and fragmentation of DNA were observed following exposure to RSVL. Co-treatment with pan-caspase inhibitor completely prevents cell death induced by RSVL. We found that RSVL-induced apoptosis correlates with sustained activation of ERK1/2 and suppression of Bcl-2 expression. Inhibition of ERK1/2 activation by its specific inhibitor or small interfering RNA reverses the effect of RSVL on Bcl-2 suppression and inhibits apoptosis, while overexpression of MEK1, which is directly upstream of both ERK1 and ERK2, enhances apoptosis induced by RSVL. Moreover, ERK1/2 was found to act upstream of caspase-3 to induce apoptosis, while it was not directly involved in caspase-3 cleavage. The other closely related MAPK members, p38 and JNK are not involved in apoptosis induced by RSVL in MDA-MB-231 cells. These results suggest that activation of ERK1/2 is required for RSVL-induced apoptosis in MDA-MB-231 cells. |
| المشرفين على المادة: | 0 (Anticarcinogenic Agents) 0 (Proto-Oncogene Proteins c-bcl-2) 0 (RNA, Small Interfering) 0 (Stilbenes) EC 2.4.2.30 (Poly(ADP-ribose) Polymerases) EC 2.7.1.- (Phosphatidylinositol 3-Kinases) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3) Q369O8926L (Resveratrol) |
| تواريخ الأحداث: | Date Created: 20080626 Date Completed: 20080908 Latest Revision: 20181201 |
| رمز التحديث: | 20221213 |
| PMID: | 18575753 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1019-6439 |
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