دورية أكاديمية
أعرض في EDS

In vitro photodynamic eradication of Pseudomonas aeruginosa in planktonic and biofilm culture.

التفاصيل البيبلوغرافية
العنوان: In vitro photodynamic eradication of Pseudomonas aeruginosa in planktonic and biofilm culture.
المؤلفون: Street CN; Ondine Biopharma Corporation, Bothell, WA, USA. cstreet@ondinebiopharma.com, Gibbs A, Pedigo L, Andersen D, Loebel NG
المصدر: Photochemistry and photobiology [Photochem Photobiol] 2009 Jan-Feb; Vol. 85 (1), pp. 137-43. Date of Electronic Publication: 2008 Jul 30.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Photobiology Country of Publication: United States NLM ID: 0376425 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0031-8655 (Print) Linking ISSN: 00318655 NLM ISO Abbreviation: Photochem Photobiol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2004->: Lawrence KS : American Society for Photobiology
Original Publication: Augusta, GA: American Society for Photobiology, <1996->
مواضيع طبية MeSH: Biofilms*, Plankton/*radiation effects , Pseudomonas aeruginosa/*physiology , Pseudomonas aeruginosa/*radiation effects
مستخلص: Photodynamic disinfection (PDD) is a nonantibiotic approach to treating drug-resistant bacterial infections. Pseudomonas aeruginosa, an opportunistic pathogen, is problematic because of its propensity to develop antibiotic resistance and its ability to secrete a protective biofilm matrix. This study examined the ability of PDD to eradicate planktonic and biofilm cultures of P. aeruginosa in vitro. Planktonic P. aeruginosa cultures were briefly exposed to a methylene blue-based photosensitizer formulation and subjected to energy doses ranging from 1.7 to 20.6 J cm(-2) using a 670 nm nonthermal diode laser. Biofilms were grown for 24 and 48 h and exposed to photosensitizer for 30 s before illumination with 13.2 or 26.4 J of energy. A single exposure of planktonic P. aeruginosa to photosensitizer at >15.5 J cm(-2) resulted in 100% eradication (>7 log(10) reduction from control), an effect that could be decreased significantly in the presence of the singlet oxygen quenchers l-tryptophan and sodium azide. Decreasing the energy dose below this threshold by varying both power density and illumination duration resulted in a dose-dependent decrease in bacterial kill. In addition, 24 h biofilm viability was reduced by 99% with single exposure and 99.9% with double exposure, while 48 h biofilm viability was reduced by >99.999% with both single and double exposures. This study shows that PDD is effective in eradicating planktonic and biofilm cultures of P. aeruginosa, supporting the concept that translation into clinical practice for indications such as otitis externa and wound disinfection is a viable option.
تواريخ الأحداث: Date Created: 20080805 Date Completed: 20090410 Latest Revision: 20090123
رمز التحديث: 20231215
DOI: 10.1111/j.1751-1097.2008.00407.x
PMID: 18673325
قاعدة البيانات: MEDLINE
الوصف
تدمد:0031-8655
DOI:10.1111/j.1751-1097.2008.00407.x