دورية أكاديمية
Designed glycopeptides with different beta-turn types as synthetic probes for the detection of autoantibodies as biomarkers of multiple sclerosis.
| العنوان: | Designed glycopeptides with different beta-turn types as synthetic probes for the detection of autoantibodies as biomarkers of multiple sclerosis. |
|---|---|
| المؤلفون: | Carotenuto A; Department of Pharmaceutical and Toxicological Chemistry, University of Naples Federico II, via D. Montesano 49, I-80131 Naples, Italy., Alcaro MC, Saviello MR, Peroni E, Nuti F, Papini AM, Novellino E, Rovero P |
| المصدر: | Journal of medicinal chemistry [J Med Chem] 2008 Sep 11; Vol. 51 (17), pp. 5304-9. Date of Electronic Publication: 2008 Aug 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 9716531 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4804 (Electronic) Linking ISSN: 00222623 NLM ISO Abbreviation: J Med Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Chemical Society Original Publication: [Easton, Pa.] : American Chemical Society, [c1963- |
| مواضيع طبية MeSH: | Drug Design* , Glycopeptides*/chemical synthesis , Glycopeptides*/immunology, Autoantibodies/*blood , Molecular Probes/*chemical synthesis , Multiple Sclerosis/*diagnosis, Antibody Affinity ; Antigen-Antibody Reactions ; Biomarkers/blood ; Humans ; Protein Structure, Secondary ; Structure-Activity Relationship |
| مستخلص: | Circulating autoantibodies have been recognized as disease biomarkers of autoimmune diseases. We have previously disclosed a synthetic glycopeptide that is able to detect specific autoantibodies in sera of patients who are affected by multiple sclerosis (MS). This glycopeptide is characterized by a type I' beta-turn around the minimal epitope Asn(Glc) that allows an efficient exposure of this moiety to antibody interactions in the context of a solid-phase immunoenzymatic assay. With the aim of optimizing the glycopeptide-antibody interactions, we analyze a series of new glycopeptides based on different turn structures. Our results confirm the role of conformation in the recognition and binding of synthetic antigenic probes to MS autoantibodies. Glycopeptide 2, which is characterized by a type I beta-turn around the minimal epitope Asn(Glc), shows the highest antibody affinity (IC50 = 11.8 nM), and thus it appears to be a promising tool for the detection of specific autoantibodies as MS biomarker in patients' sera. |
| المشرفين على المادة: | 0 (Autoantibodies) 0 (Biomarkers) 0 (Glycopeptides) 0 (Molecular Probes) |
| تواريخ الأحداث: | Date Created: 20080821 Date Completed: 20081024 Latest Revision: 20151119 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/jm800391y |
| PMID: | 18712857 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-4804 |
|---|---|
| DOI: | 10.1021/jm800391y |