دورية أكاديمية
Localization of K+-stimulated p-NPPase in the lachrymal 'salt' gland of Malaclemys, using cytochemical and autoradiographical techniques.
العنوان: | Localization of K+-stimulated p-NPPase in the lachrymal 'salt' gland of Malaclemys, using cytochemical and autoradiographical techniques. |
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المؤلفون: | Thompson IG, Cowan FB |
المصدر: | Cell and tissue research [Cell Tissue Res] 1976 Nov 10; Vol. 174 (3), pp. 417-26. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417625 Publication Model: Print Cited Medium: Print ISSN: 0302-766X (Print) Linking ISSN: 0302766X NLM ISO Abbreviation: Cell Tissue Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
مواضيع طبية MeSH: | 4-Nitrophenylphosphatase/*analysis , Lacrimal Apparatus/*enzymology , Phosphoric Monoester Hydrolases/*analysis , Salt Gland/*enzymology , Turtles/*metabolism, Adenosine Triphosphatases/metabolism ; Animals ; Autoradiography ; Biological Transport, Active ; Cell Membrane/enzymology ; Histocytochemistry ; Lacrimal Apparatus/ultrastructure ; Microscopy, Electron ; Potassium/metabolism ; Salt Gland/ultrastructure ; Sodium/metabolism |
مستخلص: | Using two independent techniques, histochemistry and autoradiography, an enzyme (E.C. 3.6.1.3.) has been localized on basolateral cell membranes of salt secreting cells in the lachrymal gland of Malaclemys. This enzyme is ouabain sensitive. In addition an L-tetramisole sensitive alkaline phosphatase is found in the same sites, and an ethacrynic acid sensitive K+-stimulated p-NPPase is found on the apical membrane. The significance of these results with regard to the location of the pump responsible for net transepithelial sodium transport is discussed. |
References: | J Cell Biol. 1973 Sep;58(3):724-30. (PMID: 4747925) J Gen Physiol. 1967 Sep;50(8):2061-83. (PMID: 6066064) J Histochem Cytochem. 1973 May;21(5):488-98. (PMID: 4269561) J Histochem Cytochem. 1972 Jan;20(1):23-38. (PMID: 4333664) Comp Biochem Physiol. 1970 Jan 15;32(2):161-74. (PMID: 5417451) Pflugers Arch. 1969;307(3):138-53. (PMID: 4238338) J Morphol. 1973 Oct;141(2):157-69. (PMID: 4748958) J Cell Biol. 1969 Feb;40(2):305-21. (PMID: 5812466) J Cell Biol. 1972 Jun;53(3):704-14. (PMID: 4554986) Comp Biochem Physiol A Comp Physiol. 1974 Dec 1;49(4):775-85. (PMID: 4154177) Biochim Biophys Acta. 1975 Jan 28;375(2):268-73. (PMID: 123762) J Histochem Cytochem. 1972 Jan;20(1):13-22. (PMID: 4333662) |
المشرفين على المادة: | 9NEZ333N27 (Sodium) EC 3.1.3.2 (Phosphoric Monoester Hydrolases) EC 3.1.3.41 (4-Nitrophenylphosphatase) EC 3.6.1.- (Adenosine Triphosphatases) RWP5GA015D (Potassium) |
تواريخ الأحداث: | Date Created: 19761110 Date Completed: 19770216 Latest Revision: 20190720 |
رمز التحديث: | 20221213 |
DOI: | 10.1007/BF00220684 |
PMID: | 187343 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0302-766X |
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DOI: | 10.1007/BF00220684 |