دورية أكاديمية

Localization of K+-stimulated p-NPPase in the lachrymal 'salt' gland of Malaclemys, using cytochemical and autoradiographical techniques.

التفاصيل البيبلوغرافية
العنوان: Localization of K+-stimulated p-NPPase in the lachrymal 'salt' gland of Malaclemys, using cytochemical and autoradiographical techniques.
المؤلفون: Thompson IG, Cowan FB
المصدر: Cell and tissue research [Cell Tissue Res] 1976 Nov 10; Vol. 174 (3), pp. 417-26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417625 Publication Model: Print Cited Medium: Print ISSN: 0302-766X (Print) Linking ISSN: 0302766X NLM ISO Abbreviation: Cell Tissue Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin, New York, Springer-Verlag.
مواضيع طبية MeSH: 4-Nitrophenylphosphatase/*analysis , Lacrimal Apparatus/*enzymology , Phosphoric Monoester Hydrolases/*analysis , Salt Gland/*enzymology , Turtles/*metabolism, Adenosine Triphosphatases/metabolism ; Animals ; Autoradiography ; Biological Transport, Active ; Cell Membrane/enzymology ; Histocytochemistry ; Lacrimal Apparatus/ultrastructure ; Microscopy, Electron ; Potassium/metabolism ; Salt Gland/ultrastructure ; Sodium/metabolism
مستخلص: Using two independent techniques, histochemistry and autoradiography, an enzyme (E.C. 3.6.1.3.) has been localized on basolateral cell membranes of salt secreting cells in the lachrymal gland of Malaclemys. This enzyme is ouabain sensitive. In addition an L-tetramisole sensitive alkaline phosphatase is found in the same sites, and an ethacrynic acid sensitive K+-stimulated p-NPPase is found on the apical membrane. The significance of these results with regard to the location of the pump responsible for net transepithelial sodium transport is discussed.
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المشرفين على المادة: 9NEZ333N27 (Sodium)
EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
EC 3.1.3.41 (4-Nitrophenylphosphatase)
EC 3.6.1.- (Adenosine Triphosphatases)
RWP5GA015D (Potassium)
تواريخ الأحداث: Date Created: 19761110 Date Completed: 19770216 Latest Revision: 20190720
رمز التحديث: 20221213
DOI: 10.1007/BF00220684
PMID: 187343
قاعدة البيانات: MEDLINE
الوصف
تدمد:0302-766X
DOI:10.1007/BF00220684