دورية أكاديمية
Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution.
| العنوان: | Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution. |
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| المؤلفون: | Ghate DA; Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 30322, USA., Holley G, Dollinger H, Bullock JP, Markwardt K, Edelhauser HF |
| المصدر: | Cornea [Cornea] 2008 Oct; Vol. 27 (9), pp. 1050-6. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 8216186 Publication Model: Print Cited Medium: Internet ISSN: 1536-4798 (Electronic) Linking ISSN: 02773740 NLM ISO Abbreviation: Cornea Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Hagerstown, MD : Lippincott Williams & Wilkins Original Publication: New York, N.Y. : Masson Pub. USA, c1982- |
| مواضيع طبية MeSH: | Therapeutic Irrigation*, Cornea/*surgery , Endothelium, Corneal/*metabolism , Mucins/*metabolism , Ophthalmic Solutions/*administration & dosage , Phacoemulsification/*methods, Aged ; Cornea/ultrastructure ; Endothelium, Corneal/drug effects ; Endothelium, Corneal/ultrastructure ; Humans ; In Vitro Techniques ; Middle Aged ; Mucins/ultrastructure ; Ophthalmic Solutions/pharmacology |
| مستخلص: | Purpose: To evaluate human corneal endothelial mucin layer thickness and ultrastructure after phacoemulsification and irrigation-aspiration with either next generation ophthalmic irrigating solution (NGOIS) or BSS PLUS. Methods: Paired human corneas were mounted in an artificial anterior chamber, exposed to 3 minutes of continuous ultrasound (US) at 80% power using the Alcon SERIES 20000 LEGACY surgical system (n = 9) or to 2 minutes of pulsed US at 50% power, 50% of the time at 20 pps using the Alcon INFINITI Vision System (n = 5), and irrigated with 250 mL of either NGOIS or BSS PLUS. A control group of paired corneas did not undergo phacoemulsification or irrigation-aspiration (n = 5). Corneas were divided and fixed for mucin staining or transmission electron microscopy. Mucin layer thickness was measured on the transmission electron microscopy prints. Results: The mucin layer thickness in the continuous phaco group was 0.77 +/- 0.02 microm (mean +/- SE) with NGOIS and 0.51 +/- 0.01 microm with BSS PLUS (t test, P < 0.001). The mucin layer thickness in the pulsed phaco group was 0.79 +/- 0.02 microm with NGOIS and 0.54 +/- 0.01 microm with BSS PLUS (P < 0.001). The mucin layer thickness in the untreated control group was 0.72 +/- 0.02 microm. The endothelial ultrastructure was normal in all corneas. Conclusions: In this in vitro corneal model, NGOIS, due to its lower surface tension and higher viscosity, preserved endothelial mucin layer thickness better than BSS PLUS with both the INFINITI Vision System (pulsed US) and the LEGACY surgical system (continuous US). |
| التعليقات: | Comment in: Cornea. 2010 Jan;29(1):121. (PMID: 19907291) |
| معلومات مُعتمدة: | EY-00933 United States EY NEI NIH HHS; P30-EY06360 United States EY NEI NIH HHS |
| المشرفين على المادة: | 0 (Mucins) 0 (Ophthalmic Solutions) |
| تواريخ الأحداث: | Date Created: 20080925 Date Completed: 20081209 Latest Revision: 20141120 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1097/ICO.0b013e31817313cb |
| PMID: | 18812770 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1536-4798 |
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| DOI: | 10.1097/ICO.0b013e31817313cb |