دورية أكاديمية
Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India.
| العنوان: | Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India. |
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| المؤلفون: | Joseph AY; Molecular Biology Laboratory, SCMS Institute of Bioscience and Biotechnology Research and Development, South Kalamassery, Cochin 682 033, India., Babu VS, Dev SS, Gopalakrishnapai J, Harish M, Rajesh MD, Anisha S, Mohankumar C |
| المصدر: | Indian journal of experimental biology [Indian J Exp Biol] 2008 Aug; Vol. 46 (8), pp. 573-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Institute of Science Communication and Information Resources, CSIR Country of Publication: India NLM ID: 0233411 Publication Model: Print Cited Medium: Print ISSN: 0019-5189 (Print) Linking ISSN: 00195189 NLM ISO Abbreviation: Indian J Exp Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New Delhi : National Institute of Science Communication and Information Resources, CSIR Original Publication: New Delhi, Council of Scientific & Industrial Research. |
| مواضيع طبية MeSH: | Alphavirus Infections/*virology , Chikungunya virus/*genetics , Chikungunya virus/*isolation & purification , Fever/*virology, Amino Acid Sequence ; Chikungunya virus/chemistry ; Humans ; India ; Molecular Biology ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Time Factors ; Viral Proteins/chemistry ; Viral Proteins/genetics |
| مستخلص: | There has been a resurgence and prevalence of fever with symptoms of Chikungunya (CHIK) and increased death toll in Kerala, the southern-most state of India. The objective of this study was to develop a rapid detection method to determine the presence of CHIK- virus in the serum samples collected from febrile patients in Kerala, India. Serum specimens were analyzed for CHIK viral RNA by RT-PCR using primers specific for nsP1 and E1 genes. Five out of twenty clinical samples were positive for CHIK virus. The partial sequences of the E1 and nsP1 genes of the strain, IndKL01 were highly similar to the Reunion strains and the recently isolated Indian strains. A novel substitution, A148V, was detected in the E1 gene of the isolate, IndKL02. The detection procedure used in this study was simple, sensitive and rapid (less than 4 hr). This result suggests that CHIK viruses similar to the Reunion strains, which had resulted in high morbidity and mortality rates, may have caused the recent Chikungunya outbreak in India. The effect of the variant, E1-A148V, in the virulence and the rate of transmission of the virus deserves further investigation. |
| المشرفين على المادة: | 0 (Viral Proteins) |
| تواريخ الأحداث: | Date Created: 20080926 Date Completed: 20090707 Latest Revision: 20080925 |
| رمز التحديث: | 20231215 |
| PMID: | 18814485 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0019-5189 |
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