دورية أكاديمية
Functional unit of 30 kDa for proximal tubule water channels as revealed by radiation inactivation.
| العنوان: | Functional unit of 30 kDa for proximal tubule water channels as revealed by radiation inactivation. |
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| المؤلفون: | van Hoek AN; Department of Physiology, University of Nijmegen, The Netherlands., Hom ML, Luthjens LH, de Jong MD, Dempster JA, van Os CH |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 1991 Sep 05; Vol. 266 (25), pp. 16633-5. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Body Water/*metabolism , Kidney Tubules, Proximal/*metabolism, Alkaline Phosphatase/metabolism ; Animals ; Cell Membrane Permeability ; Electrons ; Kidney Tubules, Proximal/enzymology ; Kidney Tubules, Proximal/radiation effects ; Kinetics ; Light ; Microvilli/enzymology ; Microvilli/metabolism ; Microvilli/radiation effects ; Molecular Weight ; Osmosis ; Rats ; Scattering, Radiation ; Temperature |
| مستخلص: | The high water permeability of kidney proximal tubules is of paramount importance for isotonic reabsorption of 70% of the glomerular filtrate, and water channels have been postulated to account for the high water permeability. Target analysis following radiation inactivation was used to probe the molecular size of the water channel. Samples of brush border membranes from rat renal cortex were subjected to 3-MeV electron pulses from the Van de Graaff accelerator at a temperature of -130 degrees C. The inactivation of the renal brush border enzymes, alkaline phosphatase, and maltase was used for internal standardization of accumulated dose measurements in target analysis of the water channel. Osmotic water permeability was measured by following the change in scattered light intensity upon rapid mixing of vesicles with a hypertonic solution using stopped-flow spectrophotometry. The vesicle shrinkage response was biphasic and the rate of the fast phase decreased dose dependently by irradiation corresponding to a target size of 30 +/- 3.5 kDa. The total change in scattered light intensity was unaltered, indicating that irradiation did not destroy the lipid barrier. Our results provide strong support for the hypothesis that the high osmotic water permeability of renal proximal tubules results from a water channel-specific protein with a functional unit of 30 kDa. |
| المشرفين على المادة: | EC 3.1.3.1 (Alkaline Phosphatase) |
| تواريخ الأحداث: | Date Created: 19910905 Date Completed: 19911004 Latest Revision: 20210210 |
| رمز التحديث: | 20231215 |
| PMID: | 1885592 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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