دورية أكاديمية
Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE).
| العنوان: | Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE). |
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| المؤلفون: | Gherghi ICh; Analytical Chemistry Laboratory, Department of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece., Girousi ST, Voulgaropoulos AN, Tzimou-Tsitouridou R |
| المصدر: | Talanta [Talanta] 2003 Oct 17; Vol. 61 (2), pp. 103-12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مستخلص: | The interaction of ethidium bromide (EB) and acridine orange (AO) with double stranded (ds), thermally denatured (ss) and supercoiled (sc) DNA, in solution, was studied by alternating current voltammetry (AC voltammetry) at the hanging mercury drop electrode (HMDE) in 0.3 M NaCl+50 mM sodium phosphate buffer (pH 8.5). Their interaction with DNA is shown to be time dependent and completely different. The changes at peak 2 (peak at -1.20 V) of dsDNA form and the appearance of peak 3 (peak at -1.42 V) in scDNA form are presented as criteria declaring the different mechanism of interaction of EB and AO with DNA. Additionally, the appearance of a new peak at around -0.44 V as a result of DNA and AO interaction, differentiates the studied behaviors. The comparison of the electrochemical behaviors of these compounds highlights the differences in the mechanism of interaction. |
| تواريخ الأحداث: | Date Created: 20081031 Date Completed: 20130704 Latest Revision: 20081030 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/S0039-9140(03)00238-8 |
| PMID: | 18969168 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
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| DOI: | 10.1016/S0039-9140(03)00238-8 |