دورية أكاديمية
Detection of antibodies against Anaplasma phagocytophilum in Algerian mice (Mus spretus), Portugal.
| العنوان: | Detection of antibodies against Anaplasma phagocytophilum in Algerian mice (Mus spretus), Portugal. |
|---|---|
| المؤلفون: | Santos AS; Centro de Estudos de Vectores e Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal. ana.santos@insa.min-saude.pt, Amaro F, Santos-Silva MM, De Sousa R, Mathias ML, Ramalhinho MG, Nuncio MS, Alves MJ, Bacellar F, Dumler JS |
| المصدر: | Vector borne and zoonotic diseases (Larchmont, N.Y.) [Vector Borne Zoonotic Dis] 2009 Dec; Vol. 9 (6), pp. 663-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 100965525 Publication Model: Print Cited Medium: Internet ISSN: 1557-7759 (Electronic) Linking ISSN: 15303667 NLM ISO Abbreviation: Vector Borne Zoonotic Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., [2001- |
| مواضيع طبية MeSH: | Anaplasma phagocytophilum/*isolation & purification , Mice/*microbiology , Rats/*microbiology, Anaplasma phagocytophilum/immunology ; Animals ; Antibodies, Bacterial/blood ; DNA Primers ; Female ; Fluorescent Antibody Technique, Indirect ; Male ; Mice/blood ; Polymerase Chain Reaction ; Portugal ; Rats/blood |
| مستخلص: | The recent detection of Anaplasma phagocytophilum in Portugal stimulated further research on the agent's enzootic cycle, which usually involves rodents. Thus a total 322 rodents belonging to five species, including 30 Apodemus sylvaticus (wood mouse), 65 Mus musculus (house mouse), 194 M. spretus (algerian mouse), 5 Rattus norvegicus (brown rat) and 28 R. rattus (black rat), were studied by indirect immunofluorescent assay (IFA) and/or polymerase chain reaction (PCR) for A. phagocytophilum exposure in four sampling areas of mainland and two areas of Madeira Island, Portugal. Overall, 3.6% (7/194) of M. spretus presented with IFA-positive results. Seropositive mice were detected in all three mainland sampling areas where this species was captured, with prevalence of 5.2% (5/96) and 5.0% (1/20) for the Ixodes-areas of Arrábida and Mafra, and 1.3% (1/78) for Mértola, a difference that was not statistically significant (p > 0.05). The majority of IFA-positive mice were detected in spring when considering either Arrábida alone (p = 0.026) or all M. spretus sampling areas together (p = 0.021), although the significance of this association was not evident after Bonferroni correction. Nevertheless, neither the seropositive M. spretus, nor additional samples of 10% seronegative rodents from mainland, and 16% of rodents collected in Madeira Island showed evidence of A. phagocytophilum active infections when spleen and/or lung samples were tested by PCR. Either the M. spretus results represents residual antibodies from past A. phagocytophilum infections, present infections with limited bacteremia, or cross-reactions with closely related agents deserves more investigation. |
| المشرفين على المادة: | 0 (Antibodies, Bacterial) 0 (DNA Primers) |
| تواريخ الأحداث: | Date Created: 20081101 Date Completed: 20100422 Latest Revision: 20140408 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1089/vbz.2008.0066 |
| PMID: | 18973448 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7759 |
|---|---|
| DOI: | 10.1089/vbz.2008.0066 |