دورية أكاديمية
MicroRNA expression in melanocytic nevi: the usefulness of formalin-fixed, paraffin-embedded material for miRNA microarray profiling.
| العنوان: | MicroRNA expression in melanocytic nevi: the usefulness of formalin-fixed, paraffin-embedded material for miRNA microarray profiling. |
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| المؤلفون: | Glud M; Department of Plastic Surgery and Burn Unit, Faculty of Health Sciences, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark. martin.glud@dadlnet.dk, Klausen M, Gniadecki R, Rossing M, Hastrup N, Nielsen FC, Drzewiecki KT |
| المصدر: | The Journal of investigative dermatology [J Invest Dermatol] 2009 May; Vol. 129 (5), pp. 1219-24. Date of Electronic Publication: 2008 Nov 13. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0426720 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1747 (Electronic) Linking ISSN: 0022202X NLM ISO Abbreviation: J Invest Dermatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : New York : Elsevier Original Publication: Baltimore, Williams & Wilkins. |
| مواضيع طبية MeSH: | Gene Expression Profiling/*methods , MicroRNAs/*metabolism , Nevus, Pigmented/*metabolism , Oligonucleotide Array Sequence Analysis/*methods , Skin Neoplasms/*metabolism, Adult ; Cryopreservation ; Female ; Formaldehyde ; Gene Expression Regulation, Neoplastic ; Histocytological Preparation Techniques ; Humans ; Male ; MicroRNAs/genetics ; Middle Aged ; Nevus, Pigmented/genetics ; Paraffin Embedding ; Skin Neoplasms/genetics |
| مستخلص: | MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate cellular differentiation, proliferation, and apoptosis. MiRNAs are expressed in a developmentally regulated and tissue-specific manner. Aberrant expression may contribute to pathological processes such as cancer, and miRNA may therefore serve as biomarkers that may be useful in a clinical environment for diagnosis of various diseases. Most miRNA profiling studies have used fresh tissue samples. However, in some types of cancer, including malignant melanoma, fresh material is difficult to obtain from primary tumors, and most surgical specimens are formalin fixed and paraffin embedded (FFPE). To explore whether FFPE material would be suitable for miRNA profiling in melanocytic lesions, we compared miRNA expression patterns in FFPE versus fresh frozen samples, obtained from 15 human melanocytic nevi. Out of microarray data, we identified 84 miRNAs that were expressed in both types of samples and represented an miRNA profile of melanocytic nevi. Our results showed a high correlation in miRNA expression (Spearman r-value of 0.80) between paired FFPE and fresh frozen material. The data were further validated by quantitative RT-PCR. In conclusion, FFPE specimens of melanocytic lesions are suitable as a source for miRNA microarray profiling. |
| المشرفين على المادة: | 0 (MicroRNAs) 1HG84L3525 (Formaldehyde) |
| تواريخ الأحداث: | Date Created: 20081114 Date Completed: 20090501 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1038/jid.2008.347 |
| PMID: | 19005486 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1523-1747 |
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| DOI: | 10.1038/jid.2008.347 |