دورية أكاديمية
Immunoresistant human glioma cell clones selected with alloreactive cytotoxic T lymphocytes: downregulation of multiple proapoptotic factors.
| العنوان: | Immunoresistant human glioma cell clones selected with alloreactive cytotoxic T lymphocytes: downregulation of multiple proapoptotic factors. |
|---|---|
| المؤلفون: | Gomez GG; The Brain Tumor Research Program, Sidney Kimmel Cancer Center, San Diego, CA 92121., Hickey MJ, Tritz R, Kruse CA |
| المصدر: | Gene therapy & molecular biology [Gene Ther Mol Biol] 2008 Jun; Vol. 12 (1), pp. 101-110. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Gene Therapy Press Country of Publication: United States NLM ID: 9815849 Publication Model: Print Cited Medium: Print ISSN: 1529-9120 (Print) Linking ISSN: 15299120 NLM ISO Abbreviation: Gene Ther Mol Biol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Palo Alto, CA : Gene Therapy Press, c1998- |
| مستخلص: | We previously reported the cellular, functional and cytogenetic characterization of immunoresistant (IR) 13-06-IR29 and 13-06-IR30 human glioma cell clones isolated after immunoselection with alloreactive cytotoxic T lymphocytes (aCTL). Relative to the 13-06-MG parental cells, both clones resisted aCTL lysis at multiple effector to target ratios; the resistant phenotype was maintained for 13-41 cell doublings after cloning and when selective pressure was removed; cross-resistance to other inducers of apoptosis/cell death was also observed (Gomez et al, 2006; Gomez and Kruse, 2007). In this study we further characterize the IR clones for factors that may contribute to the resistance. Data obtained by in-vitro quantitative morphologic and 7-amino actinomycin D flow cytometric assays revealed reduced apoptotic cell death when IR clones were coincubated with aCTL, relative to the parental cells. Since changes in apoptosis were observed, we examined the expression patterns of apoptosis-related genes in several extracts of parental cells and IR clones using pathway-specific cDNA microarray analysis. In general, the apoptotic factors were downregulated in the IR clones. From three separate extracts analyzed separately on microarrays, three factors, ATM, caspases 3 and 8, were statistically downregulated in both IR clones. Immunoblotting of the proteins confirmed the findings. Therefore, a possible mechanism for immunoresistance in gliomas may be achieved by the downregulation of one or more genes in the apoptotic pathway. |
| References: | Oncogene. 2000 Feb 17;19(7):899-905. (PMID: 10702798) Clin Cancer Res. 2005 Aug 1;11(15):5515-25. (PMID: 16061868) Cancer. 1993 Jul 15;72(2):491-501. (PMID: 8319179) Int J Radiat Oncol Biol Phys. 2001 Jun 1;50(2):511-23. (PMID: 11380241) Cell Transplant. 1992;1(4):307-12. (PMID: 1344303) Cancer Res. 2004 Mar 15;64(6):2245-50. (PMID: 15026369) Cancer Genet Cytogenet. 2006 Mar;165(2):121-34. (PMID: 16527606) Gene Ther. 2000 May;7(10):852-8. (PMID: 10845723) Nat Cell Biol. 2001 Jun;3(6):552-8. (PMID: 11389439) Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2909-14. (PMID: 8610141) Cell. 1997 Aug 8;90(3):405-13. (PMID: 9267021) J Immunol. 1997 May 15;158(10):4521-4. (PMID: 9144461) Cancer Res. 2005 Dec 15;65(24):11469-77. (PMID: 16357155) J Immunol. 1986 Jun 1;136(11):4054-62. (PMID: 2422272) Biotechnol Appl Biochem. 1997 Jun;25(3):197-205. (PMID: 9198273) J Neurooncol. 1994;19(2):161-8. (PMID: 7964992) Cancer Immunol Immunother. 1997 Oct;45(2):77-87. (PMID: 9390198) J Interferon Cytokine Res. 2003 Jul;23(7):379-93. (PMID: 14511464) Neuro Oncol. 2004 Apr;6(2):83-95. (PMID: 15134622) Hematol Oncol Clin North Am. 2001 Dec;15(6):1053-71. (PMID: 11770298) Clin Cancer Res. 2006 May 1;12(9):2716-29. (PMID: 16675563) J Neurooncol. 1999;45(2):141-57. (PMID: 10778730) Immunity. 2000 Jun;12(6):621-32. (PMID: 10894162) Cell. 1998 Sep 18;94(6):739-50. (PMID: 9753321) Gene Ther Mol Biol. 2006;10(A):133-146. (PMID: 16810329) Proc Natl Acad Sci U S A. 1990 Dec;87(24):9577-81. (PMID: 2263613) BMC Bioinformatics. 2006 Mar 02;7:106. (PMID: 16512900) Biochimie. 2002 Feb-Mar;84(2-3):203-14. (PMID: 12022951) Clin Cancer Res. 2000 Jun;6(6):2209-18. (PMID: 10873070) Cell Death Differ. 2001 Nov;8(11):1052-65. (PMID: 11687884) Brain Pathol. 2003 Oct;13(4):431-9. (PMID: 14655749) Oncogene. 2001 Sep 13;20(41):5865-77. (PMID: 11593392) N Engl J Med. 2005 Mar 10;352(10):987-96. (PMID: 15758009) J Immunother. 2007 Apr;30(3):261-73. (PMID: 17414317) Cancer Immunol Immunother. 1992;34(5):349-54. (PMID: 1371720) |
| معلومات مُعتمدة: | F31 CA094834 United States CA NCI NIH HHS; R21 NS056300 United States NS NINDS NIH HHS; R21 NS046463-01 United States NS NINDS NIH HHS; R21 NS046463 United States NS NINDS NIH HHS; R21 NS056300-01 United States NS NINDS NIH HHS; R21 NS046463-02 United States NS NINDS NIH HHS; R01 CA121258 United States CA NCI NIH HHS |
| تواريخ الأحداث: | Date Created: 20081211 Latest Revision: 20240408 |
| رمز التحديث: | 20240408 |
| مُعرف محوري في PubMed: | PMC2597650 |
| PMID: | 19066635 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-9120 |
|---|