دورية أكاديمية
Malnutrition induces an increase in intermediate filament protein content of rat cerebral cortex.
| العنوان: | Malnutrition induces an increase in intermediate filament protein content of rat cerebral cortex. |
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| المؤلفون: | Paz MM; Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil., Valente GB, Tasca CI, de Mattos AG, Pureur RP |
| المصدر: | The Journal of nutrition [J Nutr] 1991 Sep; Vol. 121 (9), pp. 1349-54. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0404243 Publication Model: Print Cited Medium: Print ISSN: 0022-3166 (Print) Linking ISSN: 00223166 NLM ISO Abbreviation: J Nutr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York, NY] : Elsevier Original Publication: 1928-1933 : Springfield, Ill. : C. C. Thomas |
| مواضيع طبية MeSH: | Cerebral Cortex/*metabolism , Intermediate Filament Proteins/*metabolism , Protein-Energy Malnutrition/*metabolism, Animals ; Animals, Newborn ; Body Weight ; Diet ; Electrophoresis, Polyacrylamide Gel ; Female ; Intermediate Filament Proteins/analysis ; Intermediate Filaments/metabolism ; Male ; Rats ; Rats, Inbred Strains |
| مستخلص: | Wistar rats were fed a normal protein (25% casein) or an isoenergetic low protein (8% casein) diet from the day of giving birth until pups were weaned. Some litters were killed at weaning; others (both normal and malnourished animals) received the 25% protein diet until d 90 when they were killed. Intermediate filament (IF) preparations were obtained by extraction of the cerebral cortex with a high salt PBS solution containing 1% Triton X-100. The pellet contained the bulk of the cytoskeleton proteins from tissue, identified as the 150- and 68-kDa subunits of neurofilaments (NF-M and NF-L, respectively), the 66-kDa associated protein, the 57-kDa intermediate filament-like protein, and the 50-kDa glial fibrillary acidic protein. Intermediate filament-enriched fractions from control and malnourished rats at both d 21 and 90 were scanned following two-dimensional gel electrophoresis to determine the effects of postnatal malnutrition on the intermediate filament protein content. The results indicated that postnatal malnutrition imposed during the brain growth spurt period did not alter the expression of IF proteins of the cerebral cortex in 21-d-old rats, but increased the expression of NF-L and NF-M proteins in adult rats. |
| المشرفين على المادة: | 0 (Intermediate Filament Proteins) |
| تواريخ الأحداث: | Date Created: 19910901 Date Completed: 19910930 Latest Revision: 20180330 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1093/jn/121.9.1349 |
| PMID: | 1908892 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-3166 |
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| DOI: | 10.1093/jn/121.9.1349 |